miR-212-5p相关ceRNA调控网络在巨噬细胞极化中的作用

Role of miR-212-5p associated ceRNA regulatory network in macrophage polarization

目的:构建巨噬细胞极化过程中竞争性内源RNA(ceRNA)调控网络,探讨其中关键lncRNA AK134492/miR-212-5p/Serpinb8轴在巨噬细胞极化中的作用。方法:构建巨噬细胞极化过程中ceRNA调控网络,通过体外功能实验筛选出其中关键轴lncRNA AK134492/miR-212-5p/Serpinb8,使用qRT-PCR、双荧光素酶报告系统及rescue实验等技术检测上述信号轴对巨噬细胞极化的调控作用。结果:体外功能实验证实miR-212-5p能促进巨噬细胞向M2极化。双荧光素酶实验证实miR-212-5p能够结合lncRNA AK134492及Serpinb8,rescue实验证实lncRNA AK134492能够调控miR-212-5p介导的靶基因Serpinb8沉默,从而影响巨噬细胞极化。结论:lncRNA AK134492通过ceRNA机制调控miR-212-5p靶基因Serpinb8的表达,影响巨噬细胞极化。

Objective:To construct a competing endogenous RNA(ceRNA)regulatory network during polarization of macrophages,and investigate the role of key regulatory axis of lncRNA AK134492/miR-212-5p/Serpinb8 in macrophage polarization.Methods:By bioinformatics analysis,we constructed the ceRNA regulatory network in macrophage polarization.Then,the key axis of lncRNA AK134492/miR-212-5p/Serpinb 8 was screened through in vitro functional assays.The regulatory effect of the lncRNA AK134492/miR-212-5p/Serpinb8 axis on macrophage polarization was detected by qRT-PCR,dual luciferase reporter system and rescue experiment.Results:In vitro functional assays confirmed that miR-212-5p promoted polarization of the macrophages shifting to M2.The Dual luciferase reporter system analysis indicated that miR-212-5p was able to bind lncRNA AK134492 and Serpinb8,and rescue experiment verification showed that lncRNA AK134492 negatively regulated the silencing of target gene Serpinb 8 mediated by miR-212-5p,thus resulting in macrophage polarization.Conclusion:Our findings suggest that lncRNA AK134492 can regulate the expression of miR-212-5p target gene Serpinb8 through the ceRNA mechanism,thereby leading to macrophage polarization.