血清miR-PC-5P-12969、miR-422b、miR-221-3p在急性缺血性脑卒中的表达及意义

Expression and significance of serum miR-PC-5P-12969,miR-422b and miR-221-3p in acute ischemic stroke

目的探讨血清miR-PC-5P-12969、miR-422b、miR-221-3p在急性缺血性脑卒中(AIS)中的表达及意义。方法收集141例初诊为AIS的患者作为研究组,按照美国国立卫生研究院卒中量表(NIHSS)评分标准将AIS患者分为轻度组、中度组和重度组。另选取同期50例无梗死病灶及无神经功能障碍的一般性心脑血管疾病患者作为对照组。采用实时荧光定量聚合酶链反应检测血清miR-PC-5P-12969、miR-422b、miR-221-3p的表达水平。采用逐步后退多因素Logistic回归分析AIS发生的危险因素,采用受试者工作特征(ROC)曲线分析3项指标诊断AIS的效能。结果轻度组、中度组、重度组AIS患者血清miR-PC-5P-12969、miR-422b、miR-221-3p表达水平明显低于对照组,且表达水平均随AIS疾病的严重程度增加呈降低趋势(P<0.05)。多因素Logistic回归分析显示血清miR-PC-5P-12969、miR-422b和miR-221-3p水平降低是AIS发生的独立危险因素(P<0.05),其比值比分别为3.025、2.573、2.304。ROC曲线分析显示血清miR-PC-5P-12969、miR-422b、miR-221-3p诊断AIS的曲线下面积分别为0.815、0.756、0.771,且有较高的特异度和灵敏度。结论血清miR-PC-5P-12969、miR-422b和miR-221-3p降低是发生AIS的独立危险因素,是早期诊断AIS、判断AIS严重程度的潜在血清学标志物。

Objective To investigate the expression and significance of serum miR-PC-5P-12969,miR-422b and miR-221-3p in acute ischemic stroke(AIS).Methods A total of 141 patients with newly diagnosed AIS were collected as the study group.According to the scoring standard of National Institutes of Health Stroke Scale(NIHSS),AIS patients were divided into mild group,moderate group and severe group.Another 50 patients without infarction and neurological dysfunction were taken as the control group.The expression of serum miR-PC-5P-12969,miR-422b and miR-221-3p were detected by real-time fluorescence quantitative polymerase chain reaction.Stepback multivariate Logistic regression was used to analyze the risk factors of AIS,and receiver operating characteristics(ROC)curve was used to analyze the value of the 3 indexes in the diagnosis of AIS.Results The expression levels of serum miR-PC-5P-12969,miR-422b and miR-221-3p in mild group,moderate group and severe group were significantly lower than those in control group,and the expression decreased with the severity of AIS disease increased(P<0.05).Multivariate Logistic regression analysis showed that the expression of serum miR-PC-5P-12969,miR-422b and miR-221-3p were independent risk factors for AIS(P<0.05),and their odds ratios were 3.025,2.573 and 2.304 respectively.ROC curve analysis showed that the areas under the curve of serum miR-PC-5P-12969,miR-422b and miR-221-3p in the diagnosis of AIS were 0.815,0.756 and 0.771 respectively,with high specificity and sensitivity.Conclusion Serum miR-PC-5P-12969,miR-422b and miR-221-3p are independent risk factors for AIS.They are potential serological markers for early diagnosis of AIS and judging the severity of AIS.