支原体肺炎患儿血清miR-222、miR-155与病情严重程度及潮气呼吸肺功能的相关研究

Relationships between serum miR-222,miR-155 levels and disease severity,tidal breathing pulmonary function in children with Mycoplasma pneumonia

目的探讨支原体肺炎患儿血清miR-222、miR-155水平与病情严重程度及潮气呼吸肺功能的关系。方法选择2019年1月至2020年12月该院收治的188例支原体肺炎患儿为研究对象,根据病情严重程度分为轻症组(n=96)和重症组(n=92),另选择同期该院体检健康儿童作为健康对照组(n=84)。采用反转录-聚合酶链反应技术检测血清miR-222、miR-155水平,并检测潮气呼吸肺功能。比较3组儿童血清miR-222、miR-155水平和潮气呼吸肺功能,分析血清miR-222、miR-155与病情严重程度、潮气呼吸肺功能的相关性,采用多元Logistic回归分析影响病情严重程度和潮气呼吸肺功能的独立危险因素。结果与健康对照组比较,轻症组、重症组儿童血清miR-222、miR-155水平明显升高,且重症组高于轻症组,差异有统计学意义(P<0.05);与健康对照组比较,轻症组、重症组潮气量(VT)、达峰时间/呼气时间(TPTEF/Te)和达峰容积/潮气量(VPTEF/VT)明显降低,呼吸频率(RR)明显升高,且重症组变化更明显,差异有统计学意义(P<0.05);血清miR-222、miR-155水平分别与VT、TPTEF/Te、VPTEF/VT呈负相关,与病情严重程度、RR呈正相关(P<0.05);多元Logistic回归分析结果显示,miR-222、miR-155水平升高为影响支原体肺炎病情严重程度和潮气呼吸肺功能的独立危险因素。结论支原体肺炎患儿血清miR-222和miR-155表达上调,且是影响病情严重程度和潮气呼吸肺功能的重要因素,可作为病情评估和预后判断的指标。

Objective To investigate the relationships between serum miR-222,miR-155 levels and disease severity,tidal breathing pulmonary function of children with Mycoplasma pneumonia.Methods A total of 188 children with Mycoplasma pneumonia in Affiliated Hospital of Inner Mongolia Medical University from January 2019 to December 2020 were enrolled,and divided into mild group(n=96),severe group(n=92)according to different severity degrees,meanwhile,other 84 healthy children were set as healthy control group.Serum miR-222 and miR-155 levels were detected by Reverse transcription-polymerase chain reaction,and tidal breathing pulmonary function was determined,then these parameters were compared between groups.The correlations between serum miR-222,miR-155 and the severity of the disease,tidal breathing pulmonary function were analyzed,and the independent risk factors affecting the severity of the disease and tidal breathing pulmonary function were analyzed by multiple Logistic regression.Results Compared with those in healthy control group,the levels of serum miR-222 and miR-155 in mild group and severe group significantly increased,and those in severe group were higher than those in mild group,the differences were statistically significant(P<0.05).Compared with those in healthy control group,tidal volume(VT),peak time to expiratory time(TPTEF/Te)and peak volume to tidal volume(VPTEF/VT)significantly decreased in in mild group and severe group,while respiratory rate(RR)significantly increased,and the changes were more obviously in severe group,the differences were statistically significant(P<0.05).Serum miR-222 and miR-155 negatively correlated with VT,TPTEF/Te and VPTEF/VT respectively,and positively correlated with disease severity and RR(P<0.05).Multiple Logistic regression analysis showed that increased miR-222 and miR-155 levels were independent risk factors affecting the severity of Mycoplasma pneumonia and tidal breathing pulmonary function.Conclusion Serum miR-222 and miR-155 levels significantly elevate in children with Mycoplasma pneumonia,and the two indicators have impacts on disease severity and tidal breathing pulmonary function,which could be used as indicators in the evaluation of patients conditions and prognosis.