超高效液相色谱法测定复方夏天无片中丹酚酸B含量

Determination of Salvianolic Acid B in Fufang Xiatianwu Tablets by Ultra Performance Liquid Chromatography

目的:建立超高效液相色谱(UPLC)法测定复方夏天无片中丹酚酸B的含量。方法:采用Phenomenex-C18色谱柱(2 mm×150 mm,3 μm),以乙腈-0.1%磷酸溶液(21∶79)为流动相;流速为0.3 mL/min;柱温35 ℃;检测波长286 nm。结果:丹酚酸B进样量在1.032 7~41.306 2 μg/mL(r=0.999 7)之间呈良好的线性关系,平均回收率为102.36%,RSD为1.11%(n=6)。结论:UPLC灵敏度高,重现性好,简便易行,可为考察复方夏天无片中丹参的质量控制提供参考。

Objective:Ultra performance liquid chromatography (UPLC) method was established for determination of salvianolic acid B in fufang xiatianwu tablets.Methods:Phenomenex C18 column (2 mm×150 mm,3 μm) was used,the mobile phase comprising of acetonitrile-0.1% phosphoric acid solution (21:79),the column temperature was 35 ℃,the velcoity was 0.3 mL/min and the detection wavelength was 286 nm.Results:The linear range of salvianolic acid B was 1.032 7 μg/mL~41.306 2 μg/mL (r=0.999 7),the average recovery was 102.36%,RSD=1.11% (n=6).Conclusion:The method has high sensitivity,good repeatability and simplicity,and can provide a reference for the quality control of radix salviae miltiorrhizae in fufang xiatianwu tablets.