摘要
目的:建立中草药制剂中41种糖皮质激素和8种抗真菌药物的超高效液相色谱-串联四级杆质谱(UPLC-MS/MS)测定方法。方法:色谱柱为Kinetex-C18(4.6 mm×50 mm,2.6 μm),梯度洗脱:流动相A为一级水,流动相B为乙腈,流速0.3 mL/min。正离子扫描,扫描方式MRM。结果:该方法有良好的线性关系,相关系数r>0.990 0,在典型的中草药制剂中,不同添加水平下方法的回收率范围为88.3%~96.1%,通过该法检出中草药制剂阳性样品3个。结论:该方法简便、快速、准确,可用于41种糖皮质激素和8种抗真菌药物的测定。
Objective:To establish an UPLC-MS/MS method for the determination of 41 glucocorticoids and 8 antifungal drugs in Chinese herbal medicine preparations.Method:A Kinetex-C18 (4.6 mm×50 mm,2.6 μm) column was used the gradient elution of water and acetonitrile as the mobile phase and the flow rate was 0.3 mL/min.The electrospray ionization source in positiveion mode was used for multiple reaction monitoring mode.Results:The method presented a good linear relation (r>0.990 0).In typical Chinese herbal medicine preparations,the recovery range of the method was 88.3%-96.1% at different levels.Three positive samples of chinese herbal medicine preparations were successfully detected by this method.Conclusion:The method was convenient,sensitive and accurate,it could be used for determination of 41 glucocorticoids and 8 antifungal drugs in Chinese herbal medicine preparations.
作者
许江红
张若良
余静
王静
王琴
刘雅茹
XU Jianghong;ZHANG Ruoliang;YU Jing;WANG Jing;WANG Qin;LIU Yalu(Ganzhou Inspection and Testing Center for Food and Drug,Ganzhou Jiangxi 341000,China)
出处
《药品评价》
CAS
2021年第21期1311-1316,共6页
Drug Evaluation