粟酒裂殖酵母中Hsp60蛋白定位与表达水平的研究

Localization and Expression of Hsp60 Protein in Schizosaccharomyces pombe

对裂殖酵母Hsp60蛋白进行生物信息学与蛋白结构分析,发现其与人和芽殖酵母分别有56%或67%的同源性和71%或79%的同源性.使用MitoProt II软件预测显示其线粒体信号肽为N端前33个氨基酸,且存在一个Chaperonin Cpn60/TCP-1 family结构域.进一步研究发现,Hsp60定位于线粒体基质.通过检测野生型细胞在30℃和37℃条件下和不同生长时期的Hsp60蛋白水平,发现Hsp60蛋白水平在37℃条件下显著增加,而在生长后期无变化.

In this paper,we analyze Hsp60 protein in Schizosaccharomyces pombe by bioinformatics and protein structure.Hsp60 shares 56%or 67%homology with human and 71%or 79%homology with budding yeast,respectively.The MitoProt II software predicts that the Mitochondria targeting sequence is the first 33 amino acids of N-terminal and that there is a Chaperonin Cpn60/TCP-1 family domain.We further find that Hsp60 is localized in the mitochondrial matrix.The Hsp60 protein level of wild-type cells at 30℃and 37℃and different growth stages are detected.It is found that the Hsp60 protein level increases significantly at 37℃,but does not change at the stationary phase.