东海原甲藻和中肋骨条藻适宜生长条件及叶绿素荧光特性的对比

Comparision on the suitable growth conditions and chlorophyll fluorescence characteristics of Prorocentrum donghaiense and Skeletonema costatum

为探究东海原甲藻(Prorocentrum donghaiense)和中肋骨条藻(Skeletonema costatum)两种赤潮高发藻适宜生长条件及叶绿素荧光参数的差异,选取温度(17、20、23、26℃)、光照[40、80、120、160μmol photons/(m^(2)·s)]、磷酸盐(0.1、1.0、5.0、10.0μmol/L)、初始生物量(1×10^(4)、3×10^(4)、5×10^(4)、7×10^(4)cells/mL)等4个因素4水平试验进行室内培养实验。结果表明,对两种微藻叶绿素a峰值质量浓度产生极其显著影响的是磷酸盐(P<0.01),光照、温度、初始生物量未产生显著影响。磷酸盐添加表明,在0.1、1.0μmol/L低磷环境中,东海原甲藻生物量及稳定性高于中肋骨条藻,高初始生物量消耗磷酸盐后东海原甲藻能够继续生存,中肋骨条藻则细胞丰度持续衰落;在5.0和10.0μmol/L高磷环境中,中肋骨条藻较东海原甲藻增殖最快,生物量更高。东海原甲藻的荧光参数F_(v)/F_(m)、α较中肋骨条藻稳定,表明其光合活性稳定;更小的细胞体积和更大的比表面积使中肋骨条藻于培养初期快速达到峰值,表明其对磷酸盐利用能力强。10.0μmol/L磷酸盐浓度组,4个荧光参数最大,光合活性最强,更容易发生赤潮。东海原甲藻的光合活性主要受光照的影响,中肋骨条藻的光合活性更大程度受磷酸盐、光照的影响。

In order to explore the suitable growth conditions and chlorophyll fluorescence parameters of two red algae Prorocentrum donghaiense and Skeletonema costatum,temperature(17,20,23,26 ℃)and light(40,80,120,160 μmol photons/(m^(2)·s)),phosphate(0.1,1.0,5.0,10.0 μmol/L),initial biomass(1×10^(4),3×10^(4),5×10^(4),7×10^(4) cells/mL),the algae culture experiment of the 4 factors with 4 levels was carried out.The results showed that the phosphate concentration(P<0.01) had the most significant effect on the peak chlorophyll a concentration of the two algae.And it was not significantly affected by light,temperature,and initial biomass.Phosphate addition experiment showed that in the low phosphate environment of 0.1 μmol/L and 1.0 μmol/L,the biomass and its stability of Prorocentrum donghaiense were higher than that of Skeletonema costatum.Prorocentrum donghaiense can survive after the high initial biomass depleting the phosphate,while the cell abundance of Skeletonema costatum declined continuously.In the environment of 5.0 and 10.0 μmol/L high phosphate,Skeletonema costatum proliferated faster and with higher biomass than Prorocentrum donghaiense.The fluorescence parameters F_(v)/F_(m) and α of Prorocentrum donghaiense were more stable than those of Skeletonema costatum,which showed the photosynthetic activity of Prorocentrum donghaiense was also stable.The smaller cell volume and larger specific surface area made Skeletonema costatum reach the peak value of biomass rapidly in the early stage of culture,which indicates that it has strong phosphate utilization ability.In the 10.0 μmol/L phosphate group,the four fluorescence parameters were the largest and the photosynthetic activity was the strongest,so it is necessary to prevent and control eutrophication.The photosynthetic activity of Prorocentrum donghaiense was more affected by light,and the photosynthetic activity of Skeletonema costatum was more affected by phosphate and light.