苦参碱对放射性肺损伤大鼠氧化应激抑制作用的实验研究

Experimental Study on the Inhibitory Effect of Matrine on Oxidative Stress in Rats with Radiation Lung Injury

目的:研究苦参碱对放射性肺损伤SD大鼠肺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化酶(GSH-PX)活性的影响,探讨苦参碱对放射性肺损伤大鼠氧化应激的抑制作用及其机制。方法:将144只健康雄性SD大鼠随机分配到正常对照组,模型组(单纯照射组),地塞米松组,苦参碱低、中、高浓度治疗组(50、100、200 mg·kg^(-1)·d^(-1)),每组24只。然后对大鼠进行肺部照射,构建大鼠放射性肺损伤实验模型,各组大鼠分别在照射完成第2、4、8、12周随机选取6只大鼠,取其血清,采用比色法检测大鼠血清MDA、SOD和谷胱甘肽过氧化酶GSH-PX的活性。结果:和单纯照射组相比,地塞米松激素组、苦参碱组(50、100、200 mg/kg)SD大鼠肺组织血清MDA含量下降,SOD活性和GSHPX活性有所升高。结论:中药苦参碱能够降低放射性肺损伤SD大鼠肺组织中MDA的含量,提高SOD活性和GSH-PX活性,从而抑制了放射性肺损伤大鼠的氧化应激反应。

Objective:To investigate the oxid ative stress inhibition effect of bit rine on radioactive lung injury in SD rats.Methods:60 healthy male SD rats were casually assigned to normal control group,model control group(simple irradiation group),dexamethasone group,matrine low-concentration group(50 mg·kg^(-1)·d^(-1)),medium-concentration group(100 mg·kg^(-1)·d^(-1))and high-concentration group(200 mg·kg^(-1)·d^(-1))rats in each group.The rat lung injury model was constructed by lung irradiation.Six rats were randomly selected at the 2nd,4th,8th and 12th weeks after irradiation.The contents of malondialdehyde and the activities of superoxide dismutase and glutathione peroxidase were detected by colorimetry.Results:When compared with irradiation group,dexamethasone hormone groups and matrine groups(50,100,200 mg/kg)decreased serum MDA and enhanceed SOD activity and GSH-PX activity in acute lung injury.Conclusion:matrine,a traditional chinese medicine,can reduce rats with radiation lung injury,improve the SOD activity and GSH-PX activity,thus inhibit the oxidative stress response of rats with radiation lung injury.