整合素β3在牦牛繁殖周期各阶段输卵管不同部位中的表达研究

Expression of Integrin β3 in Different Parts of Fallopian Tube in Yak at Different Stages of Reproduction Cycle

整合素β3作为整合素超家族的一员,在早期胚胎发育和胚胎附植中发挥着重要作用。为探究牦牛繁殖周期各阶段输卵管不同部位中整合素β3的表达情况,本研究采集繁殖周期不同阶段(卵泡期、黄体期和妊娠期)雌性牦牛的输卵管组织,按照输卵管不同部位(峡部、壶腹部和漏斗部)将试验材料分为九组,通过实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹(WB)检测整合素β3基因和蛋白在繁殖周期各阶段输卵管不同部位中的表达水平,运用免疫组织化学法对整合素β3进行定位。结果显示,在繁殖周期各阶段中,输卵管峡部的整合素β3表达量均显著高于壶腹部和漏斗部。在峡部中,整合素β3的表达量在妊娠期最高,黄体期最低;而在壶腹部和漏斗部中,整合素β3的表达量在卵泡期最高。免疫组织化学结果显示,整合素β3主要分布于牦牛输卵管的肌层、浆液腺、基细胞、黏膜上皮的分泌细胞、纤毛细胞中。综上所述,整合素β3在牦牛繁殖周期各阶段输卵管的不同部位均有表达,且存在表达差异,由此推测整合素β3对牦牛受精以及早期胚胎的发育发挥着重要作用。本研究结果为进一步探索整合素β3对牦牛生殖繁育的作用提供了理论依据。

Integrin β3 is a member of integrin superfamily, which plays an important role in early embryo development and embryo attachment. In order to explore the expression of integrin β3 in different parts of fallopian tubes at different stages of yak reproductive cycle, the fallopian tubes tissues of female yaks at different stages of reproductive cycle(follicular, luteal and pregnancy phase) were collected and divided into nine groups according to isthmus, ampullary and infundibulum. The expression levels of integrin β3 gene and protein in different parts of fallopian tubes at different stages of reproductive cycle were detected by real-time fluorescence quantitative PCR(qRT-PCR) and Western-blotting(WB), and integrin β3 was localized by immunohistochemistry. The results of qRT-PCR and WB showed that the expression of integrin β3 in isthmus was significantly higher than that in ampullary and infundibulum at different stages of reproductive cycle. In isthmus, the expression of integrin β3 was the highest in pregnancy phase and the lowest in luteal phase. In ampullary and infundibulum, the expression of integrin β3 was the highest in follicular phase. Immunohistochemical results showed that integrin β3 was mainly distributed in muscularis, serous gland, basal cells, secretory cells and ciliated cells of mucous epithelium of yak fallopian tubes. In conclusion, integrin β3 is expressed in different parts of fallopian tubes at different stages of yak reproductive cycle, and there are differences in its expression. It is speculated that integrin β3 plays an important role in yak fertilization and early embryo development, which provides a theoretical basis for further exploring the role of integrin β3 in yak reproductive breeding.