结直肠腺瘤中的miR-218-5p表达研究

Expression of miR-218-5p in colorectal adenoma

目的检测miR-218-5p在结直肠腺瘤(CRA)中的表达,并分析其临床意义。方法选取2011年1月至2013年12月上海市同济医院的16例CRA及8例非腺瘤(NA)石蜡组织作为研究对象,利用实时定量PCR(qRT-PCR)检测miR-218-5p表达,统计分析不同性别、年龄、尺寸、位置、数量、病理类型及是否伴发同时性结直肠癌(sCRC)等亚组腺瘤中的miR-218-5p表达差异。结果CRA与NA两组miR-218-5p表达比较,差异无统计学意义(P>0.05);亚组分析发现,锯齿状腺瘤(SA)组的miR-218-5p表达低于NA组,差异有统计学意义(P<0.05)。进一步分析显示,miR-218-5p在不同性别、年龄、尺寸、位置、数量及病理类型中的表达差异无统计学意义;伴发sCRC的miR-218-5p表达低于不伴sCRC的CRA,差异有统计学意义(P<0.05)。结论miR-218-5p在SA以及伴发sCRC的腺瘤组织中表达下调。

Objective To detect the expression of miR-218-5p in colorectal adenoma(CRA)and analyze its clinical significance.Methods A total of 16 cases of CRA paraffin tissues and eight cases of non-adenoma(NA)paraffin tissues from Shanghai Tongji Hospital from January 2011 to December 2013 were selected as the research objects.The expression of miR-218-5p was detected by quantitative real-time PCR(qRT-PCR).The differences in the expression of miR-218-5p in subgroups of adenomas,such as different gender,age,size,location,number,pathological type,and whether it is accompanied by simultaneous colorectal cancer(sCRC)were statistically analyzed.Results There was no significant difference in the expression of miR-218-5p between the CRA and NA groups(P>0.05).Subgroup analysis found that the expression of miR-218-5p in the serrated adenoma(SA)group was lower than that in the NA group with statistical significance(P<0.05).Further analysis showed that the expressions of miR-218-5p in different genders,ages,sizes,locations,numbers and pathological types were not statistically significant;the expression of miR-218-5p with sCRC was lower than that in CRA without sCRC with statistical significance(P<0.05).Conclusion The expression of miR-218-5p is downregulated in SA and adenoma tissues with sCRC.