维生素D受体ApaⅠ和BsmⅠ基因多态性与良性阵发性位置性眩晕发病风险探讨

The role of vitamin D receptor Apa I and BsmI gene polymorphism in the pathogenesis of benign paroxysmal positional vertigo

目的探讨维生素D受体ApaⅠ和BsmⅠ位点基因多态性与良性阵发性位置性眩晕(BPPV)发病风险相关性。方法选取2020年3月至2021年3月本院神经内科门诊的98例BPPV患者作为BPPV组, 同时选取本院体检中心体检健康的患者100例为对照组, 运用聚合酶链式反应-限制性片段长度多态性方法(PCR-RELP)检测ApaⅠ(rs7975232)和BsmⅠ(rs1544410)基因多态性, 二元Logistic回归分析BPPV发病影响因素。结果 BPPV组中的总胆固醇和血尿酸水平高于对照组(P<0.05);BPPV组和对照组的ApaⅠ、BsmⅠ基因分布的实际值和预测值对比, 差异无统计学意义(P>0.05), BPPV组和对照组的ApaⅠ、BsmⅠ基因型稳定, 符合Hardy-Weinberg平衡定律;ApaⅠ和BsmⅠ基因分布结果显示, BPPV组和对照组ApaⅠ基因rs7975232分布和BsmⅠ基因rs1544410分布差异均有统计学意义(均P<0.05);总胆固醇、血尿酸、rs7975232CC型、rs7975232AA型、rs1544410 CC型、rs1544410 CT型是发生BPPV的危险因素。结论维生素D基因限制性内切酶位点的基因多态性ApaⅠ rs7975232、BsmⅠ基因rs1544410与BPPV的发生具有一定的相关性, ApaⅠrs7975232中CC型、AA型和rs1544410 CC型、CT型人群比较容易发生BPPV。

Objective To explore the relationship between the vitamin D receptor Apa I and Bsm I gene polymorphisms and the risk of benign paroxysmal positional vertigo(BPPV).Methods The 98 patients with BPPV admitted to the Neurology Clinic of our hospital from March 2020 to March 2021 were selected as vertigo group,and 100 healthy individuals were selected as a healthy control group.The gene polymorphism of Apa I(rs7975232)and Bsm I(rs1544410)were detected by polymerase chain reaction based restriction fragment length polymorphism(PCR-RFLP).Binary Logistic regression was used to analyze the influencing factors of benign paroxysmal positional vertigo.Results The serum levels of total cholesterol and urie acid were significantly higher in the BPPV group than in a healthy control group(P<0.05).The actual and predicted values of the ApaI and Bsm I gene distribution were compared between the BPPV group and a healthy control group.and the difference was not statistically significant(P>0.05).The ApaI and Bsm I genotypes of the BPPV group and the control group were stable and consistent with the Hardy-W einberg equilibrium law.The Apa I gene rs7975232 and the Bsm I gene rs1544410 genotype and allele frequency distribution[n(%)]showed statistically significant difference(all P<0.05)between control vs BPPV groupa.The locus genotype and allele frequency distribution[n(%)]showed statistically significant difference between control vs BPPV group(P<0.05).The total cholesterol,blood uric acid,rs7975232CC,.rs7975232AA,rs1544410 CC.and rs1544410 CT were risk factors for the occurrence of benign paroxysmal positional vertigo..Conclusions Gentic polymorphisms at the cleavage sites of restriction endonuclease of vitamin D gene Apa I rs7975232 and BsmI gene rs1544410 have certain correlation with the occurrence of(BPPV)disease.Population with type CC and AA in Apal rs7975232 and type CC and CT in Bsm I rs1544410 are more prone to BPPV.