替吉奥对胃癌前病变胃黏膜超微结构及其相关蛋白影响

Effect of tegafur on ultrastructure of gastric mucosa and related proteins in precancerous lesions of gastric cancer

[目的]分析替吉奥对胃癌前病变动物模型胃黏膜组织超微结构及肿瘤组织中Bcl-2及Bax蛋白表达的影响。[方法]将40只SD大鼠随机分为空白组(10只)、模型组(15只)和观察组(15只),模型组和观察组使用MNNG饮用液造模;造模成功后空白组和模型组使用蒸馏水干预,观察组使用替吉奥灌胃每,连续灌胃给药10 w;采用常规HE染色,观察各组胃黏膜组织超微结构;使用Western Blot法检测各组大鼠组织中Notch1、Delta样配体4(DLL4)、Jagged、Bcl-2、Bax及β-actin蛋白水平。[结果]观察组胃黏膜光泽度欠佳,呈粉红色,上皮组织见良性反应增生,腺体排列较模型组更为整齐,且腺体形态规则;观察组胃粘膜组织中Notch1、DLL4和Jagged蛋白水平明显低于模型组[(0.47±0.08) vs(0.78±0.11),(0.41±0.09) vs(0.81±0.13),(0.44±0.07) vs(0.79±0.11)],且差异存统计学意义(P <0.05);观察组胃粘膜组织中Bcl-2蛋白明显低于模型组[(0.49±0.08) vs(0.68±0.13)],Bax蛋白明显高于模型组[(0.68±0.13) vs(0.34±0.07)],且差异存统计学意义(P <0.05)。[结论]替吉奥通过影响Notch信号通路,调控Bcl-2及Bax蛋白表达来改善胃癌前病变动物模型胃黏膜组织超微结构。

[Objective]To analyze the effect of tegafur on the ultrastructure of gastric mucosa and the expression of Bcl-2 and Bax protein in gastric precancerous lesions animal model. [Method] 30 rats were randomly divided into blank group,model group and observation group. The model group and the observation group were made with MNNG drinking liquid;after successful modeling,the blank group and model group were intervened with distilled water,while the observation group was given tegafur;the ultrastructure of gastric mucosa was observed by routine he staining;and Western Blot was used to observe the ultrastructure of gastric mucosa in each group. The protein levels of Notch1,DLL4,jagged,Bcl-2,Bax and β-actin were detected by Blot.[Result] The glossiness of gastric mucosa in the observation group was poor,pink,benign hyperplasia was seen in the epithelial tissue,and the gland arrangement was more orderly than that in the model group,and the gland morphology was regular;The protein levels of Notch1,DLL4 and jagged in the observation group were significantly lower than those in the model group[( 0. 47 ± 0. 08) vs( 0. 78 ± 0. 11),( 0. 41 ± 0. 09) vs( 0. 81 ± 0. 13),( 0. 44 ± 0. 07) vs( 0. 79 ± 0. 11) ],and the difference was statistically significant( P < 0. 05);the protein level of Bcl-2 in the observation group was significantly lower than that in the model group[( 0. 49 ± 0. 08) vs( 0. 68 ± 0. 13) ],and the protein level of Bax was significantly lower than that in the model group[( 0. 68 ± 0. 13) vs( 0. 34 ± 0. 07) ]. The difference was statistically significant( P < 0. 05). [Conclusion] tegafur can effectively improve the ultrastructure of gastric mucosa and regulate the expression of Bcl-2 and Bax protein by regulating Notch signaling pathway.