慢生型大豆根瘤菌HW-05高密度发酵条件优化

Optimization of high-cell-density fermentation conditions cultivation of Bradyrhizobium japonicurm HW-05

[目的]探究慢生型大豆根瘤菌HW-05高密度发酵最佳条件。[方法]采用响应面法优化HW-05培养基配方,在20 L全自动发酵罐中采用控制补料和流加的方法实现大豆根瘤菌的高密度发酵。[结果]获得大豆根瘤菌HW-05最适培养基配方为甘露醇9.45 g/L、酵母粉0.5 g/L、K_(2)HPO_(4)0.38 g/L、MgSO_(4)0.2 g/L、NaCl 0.1 g/L,此时最佳有效活菌数为3.17316×10^(9)cfu/mL,通过全自动发酵罐补料方式,有效活菌数达1.28×10^(10)cfu/mL。[结论]该菌株实现高密度发酵,有效活菌数显著提高,为根瘤菌剂应用提供基础数据。

[Objective]Exploring of the high-cell-density cultivation of Bradyrhizobium japonicurm HW-05.[Method]Optimize the HW-05 fementation medium by the response surface methodology,high-cell-density cultivation of Bradyrhizobium japonicurm HW-05 used by controll feeding in a 20 litres fully automatic fermenter. [Result]The optimal fementation medium for HW-05 is mannitol 9. 45 g/L,yeast powder 0. 5 g/L,K_(2)HPO_(4)0. 38 g/L,MgSO_(4)0. 2 g/L,NaCl 0. 1 g/L,the effective number of viable bacteria 3. 17316 × 10^(9)cfu/m L,the meyhod by automatic fermenter feeding,the effective number of viable bacteria 1. 28 × 10^(10)cfu/m L.[Conclusion]The high-cell-density cultivation of HW-05 strain was realized,the number of effective viable bacteria was increased significantly,and it provided the basic data for the application of rhizobium.