慢性乙型肝炎患者高敏HBV-DNA定量与HBeAg、HBeAb水平相关性研究

A Study on the Correlation Between High-Sensitivity HBV-DNA Quantification and HBeAg and HBeAb Levels in Patients Who Suffered Chronic Hepatitis B

目的分析慢性乙型肝炎患者HBV-DNA定量与HBeAg、HBeAb水平的相关性。方法选取本院2020年7月至2021年7月收治的慢性乙型肝炎患者4439例[其中HBeAg、HBV-DNA双(+)组1116例;HBeAb、HBV-DNA双(+)组1098例;HBeAg(+)、HBV-DNA(-)组1138例;HBeAg(-)、HBeAb(+)、HBV-DNA(+)组1087例]为研究对象,检测其外周血HBV-DNA、HBeAg、HBeAb水平并进行统计学分析。结果HBV-DNA与HBeAg水平呈正相关(r=0.569,P<0.001);依据HBV-DNA水平分为低表达组、中表达组、高表达组,3组HBeAg的S/CO值分别为222.84±35.70、907.33±59.81、1087.76±52.47,组间比较差异均有统计学意义;当HBeAg的cut-off值为49.13时,其ROC曲线下面积最大(AUC=0.780,P<0.01),其灵敏度和特异性分别是65.59%和80.14%;HBeAg阴性而HBV-DNA≥10^(5)的患者占研究对象的7.5%。结论当HBeAg水平升高时,外周血中HBV-DNA水平将会升高,在实际工作中,HBeAg可辅助判断病毒复制状况。特别关注HBeAg阴性高病毒血症患者,这可能是预后不良的表现。

Objective To analyze the correlation between HBV-DNA quantification and HBeAg,HBeAb levels in patients who suffered chronic hepatitis B.Methods A total of 4,439 cases who admitted to our hospital from July,2020 to July,2021 were selected for the study[in which 1116 cases as the HBeAg and HBV-DNA double positive group,1098 cases as the HBeAb and HBV-DNA double positive group,1138 cases as HBeAg(+)and HBV-DNA(-)groups,and 1087 cases as HBeAg(-),HBeAb(+)and HBV-DNA(+)groups].Data was processed through statistical methods.Results HBV-DNA and HBeAg levels were positively correlated(r=0.569,P<0.001);According to HBV-DNA levels,patients were divided into the low expression group,medium expression group,and high expression group.S/CO values of the three groups of HBeAg were 222.84±35.70,907.33±59.81,and 1087.76±52.47,respectively,and the differences among groups were statistically significant;When the cut-off value of HBeAg was 49.13,the area under the ROC curve was the largest(AUC=0.780,P<0.01).The sensitivity and specificity were 65.59%and 80.14%,respectively;Patients with HBeAg negative but the value of HBV DNA greater than the fifth power was up to 7.5%in all objects.Conclusion With the level of HBeAg becoming higher,the level of HBV-DNA in peripheral blood will increase correspondingly.In clinical practice,HBeAg can help to judge the status of virus replication.Special attention on patients suffered HBeAg-negative hyperviremia should be made,which may be a sign of a poor prognosis.