多巴胺对出血性脑卒中大鼠胶质纤维酸性蛋白和脑源性神经营养因子表达的影响及保护机制

Effect of dopamine on the expression of GFAP and BDNF in rats with hemorrhagic stroke and protective mechanism

目的:探讨多巴胺(DA)对出血性脑卒中大鼠的胶质纤维酸性蛋白(GFAP)和脑源性神经营养因子(BDNF)表达的影响及保护机制。方法:建立出血性脑卒中大鼠模型,将大鼠随机分为假手术组、脑出血模型组(ICH组)和DA组(脑出血模型给予DA组)。分别比较3组大鼠的神经功能障碍评分、神经元形态、脑水肿、细胞凋亡和脑组织形态变化;免疫组织化学染色法检测脑组织中GFAP和BDNF蛋白的表达。结果:与假手术组相比较,ICH组神经功能障碍评分显著降低;与ICH组相比,DA组神经功能障碍评分显著升高。与假手术组比较,ICH组尼氏小体数量显著减少;与ICH组相比,DA组尼氏小体数量明显增加;ICH组脑组织含水量和细胞凋亡数量显著高于假手术组;与ICH组相比,DA组脑组织含水量和细胞凋亡数量均显著降低。ICH组脑组织出现了不同程度的水肿,其中一部分神经细胞出现了明显肿胀,间质之间明显增宽,神经元结构受到了严重破坏,严重的神经元出现坏死,且有大量的炎性细胞浸润;DA组大鼠的脑组织形态得到明显的改善。与假手术组相比,ICH组脑组织中GFAP蛋白阳性表达显著增加,BDNF蛋白阳性表达显著降低;与ICH组大鼠相比,DA组脑组织中GFAP阳性表达明显减少,BDNF蛋白阳性表达明显增多。结论:DA能抑制脑组织中GFAP表达,促进BDNF的表达,改善脑出血的神经功能障碍,进而对出血性脑卒中受损脑组织起到保护作用。

Objective : To investigate the effect of dopamine(DA)on expression of glial fibrillary acidic protein(GFAP) and brain-derived neurotrophic factor(BDNF) in rats with hemorrhagic stroke and its protective mechanism. Methods : Rats with hemorrhagic stroke were randomly divided into sham group, intracerebral hemorrhage(ICH) model group(ICH group) and DA group(cerebral hemorrhage model group treated with DA). Neurological dysfunction score, neuron morphology, brain edema, cell apoptosis and brain tissue morphology were compared among the three groups. The expression of GFAP and BDNF protein in brain tissue was detected by immunohistochemical staining. Results : Compared with sham group, the scores of neurological dysfunction in ICH group were significantly reduced. Compared with ICH group, the neurological dysfunction score of DA group was significantly increased. Compared with the sham group, the number of Nissl bodies in ICH group was significantly decreased. Compared with ICH group, the number of Nissl bodies in DA group was significantly increased. The water content and the number of cell apoptosis in ICH group were significantly higher than those in sham group. Compared with ICH group, the water content of brain tissue and the number of apoptosis in DA group were significantly decreased. The brain tissue morphology of ICH group rats showed different degrees of edema, a part of the nerve cells showed significant swelling, the interstitial width was significantly widened, the neuron structure was seriously damaged, and severe neuron showed necrosis with a large number of inflammatory cell infiltration. The morphology of brain tissue in DA group was significantly improved. Compared with sham group, the expression of GFAP protein in ICH group was significantly increased, and the expression of BDNF protein in ICH group was significantly decreased. Compared with ICH group, the expression of GFAP in DA group was significantly decreased, and the expression of BDNF protein was significantly increased. Conclusion : DA can inhibit the expression of GFAP in brain tissue, promote the expression of BDNF, improve the neurological dysfunction after cerebral hemorrhage, and then play a protective role in the damaged brain tissue of hemorrhagic stroke.