在全自动生化分析仪中增加血清指数检测功能的设计与应用

Design and application of serum index detection function in automatic chemistry analyzer

目的在全自动生化分析仪中增加血清指数(SI)检测功能的设计,准确测定标本的SI,客观反映血清标本的脂血、溶血和黄疸程度。方法依据脂血、溶血、黄疸干扰物对波长具有选择性这一理论,设计SI检测流程,设置SI检测软件功能,并对该功能进行测试。结果采用单试剂终点法,试剂为生理盐水,选择450、505、570、600、630和700 nm 6个波长来确定标本SI,完成软件修改后,进行上机测试。通过多组样本测试,黄疸指数、溶血指数以及脂血指数的批内结果分别为11.36 mg/L、245.61 mg/L、104.30 mg/L,批内变异系数(CV)分别为1.04%、1.38%、1.27%;黄疸指数、溶血指数和脂血指数的批间结果分别为13.26 mg/L、256.32 mg/L、109.74 mg/L,批间CV分别为1.96%、2.21%、2.13%。批内CV<2.00%,批间CV<3.00%,因此SI判断溶血、黄疸和脂血满足临床要求。结论不同测试试剂受溶血、脂血、黄疸的干扰程度不同,实验室应根据检测项目设置SI界限,从而客观反映标本质量。

Objective To add the design of serum index(SI)detection function to the automatic chemistry analyzer,and accurately determine the SI of the specimen,which could objectively reflect the degree of lipemia,hemolysis and icterus of the serum specimen.Methods According to the theory that lipemia,hemolysis and icterus disruptors were selective to wavelength,the SI detection process was designed and the software function of SI detection was set and tested.Results The test used a single-reagent end-point method,which used normal saline as the reagent and chose six wavelengths of 450,505,570,600,630 and 700 nm to determine the SI.After completing the software modification,the computer test was conducted.Through the test of multiple groups of samples,the intra batch results of jaundice index,hemolysis index and lipemia index were 11.36 mg/L,245.61 mg/L and 104.30 mg/L,respectively,and the intra batch coefficients of variation(CV)were 1.04%,1.38%and 1.27%,respectively.The inter batch results of jaundice index,hemolysis index and lipemia index were 13.26 mg/L,256.32 mg/L and 109.74 mg/L,respectively,and the inter batch CV values were 1.96%,2.21%and 2.13%,respectively.The intra batch CV was less than 2.00%,and inter batch CV was less than 3.00%,so the SI judged that hemolysis,jaundice and lipemia met the clinical requirements.Conclusions Different test reagents are disturbed by hemolysis,hyperlipemia and jaundice to varying degrees.The laboratory should set SI limits according to the test items,so as to objectively reflect the quality of samples.