胃息肉患者胃液微生态分析与功能预测

High throughput sequencing and bioinformatic analysis of gastric microbiota in gastric juice of patients with gastric polyps

目的探讨幽门螺杆菌(Helicobacter pylori,Hp)对胃息肉患者胃液微生态的影响。方法纳入接受胃镜检查及Hp检测的患者。胃镜检查下胃黏膜未见异常且Hp阴性者为健康组,胃息肉者为疾病组。胃息肉者分为息肉Hp(+)组与息肉Hp(-)组。留取胃液,进行16SrRNA基因测序及生物信息学分析,对比不同组间菌群和功能基因之间的差异。结果 (1)健康组、息肉Hp(-)组和息肉Hp(+)组的胃液菌群α多样性无明显差异,β多样性差异有统计学意义(P <0.05);(2)在属水平上,息肉Hp(+)组比息肉Hp(-)组Lachnoanaerobaculum、Oribacterium丰度升高;息肉Hp(-)组比息肉Hp(+)组需氧菌丰度显著升高(P <0.05);(3)健康组、息肉Hp(-)组及息肉Hp(+)组菌群在表征网络的平均聚类系数等网络拓扑结构参数存在差异。结论幽门螺杆菌感染扰乱了胃微生态平衡,使胃息肉患者胃液微生态发生紊乱,可能影响细菌-细菌的相互作用以及细菌-宿主的作用。

Objective To investigate the effect of Hp on gastric juice microbiota in patients with gastric polyps. Methods Patients undergoing gastroscopy and Hp were selected. Patients with no abnormality of gastric mucosa and negative Hp under gastroscopy were assigned to healthy group,and patients with gastric polyps were assigned to case group. Gastric polyps were divided into polyp Hp(+)group and polyp Hp(-)group. Gastric juice was taken for 16 SrRNA Gene Sequencing and bioinformatics analysis to compare the differences of microbiota and functional genes among different groups. Results There were no significant difference in α diversity and significant differences in β diversity among the three groups(P < 0.05). At the genus level,the abundance of Lachnoanaerobaculum and Oribacterium in polyp Hp(+)group was higher than that in polyp Hp(-)group. The abundance of aerobic bacteria in polyp Hp(-)group was significantly higher than that in the polyp Hp(+)group(P < 0.05).There were differences among the three groups of microbiota in network topology parameters such as average clustering coefficient. Conclusion Hp infection disturbed the gastric microecological balance and made the gastric microbiota of gastric polyps disordered,which may affect the interaction between bacteria and bacteria and the role of bacteria host.