异甘草素抗腹泻活性及其分子机制研究

Study on the anti-diarrhea activity of isoliquiritigenin and its molecular mechanism

为确定异甘草素抗腹泻作用及其抗腹泻分子机制,利用蓖麻油诱导小鼠腹泻模型评价异甘草素抗腹泻活性,观察并记录初次腹泻时间、腹泻次数及肠道碳粉推进比等相关指标。通过网络药理学方法分析异甘草素治疗腹泻关键靶标,利用分子对接技术及荧光定量PCR确定异甘草素对靶标的影响。结果表明,灌胃异甘草素显著降低小鼠腹泻次数,延缓初次腹泻时间,减慢肠道蠕动。网络药理学研究表明,MAPK14、NCOA2、PPARG和ESR1可能是异甘草素治疗腹泻关键靶点,异甘草素下调上述基因表达,分子对接表明异甘草素可与关键蛋白结合,是一种潜在抑制剂。

In order to determine the anti-diarrhea effectand molecular mechanismof isoglycyrr hizin,the anti-diarrhea activity of isoglycyrrhizin was evaluated by using the mouse diarrhea model induced by castor oil,first diarrhea time,diarrhea number and intestinal carbon powder propulsion ratio were observed and recorded.Then,key targets of isoglycyrrhizin in the treatment of diarrhea were analyzed by network pharmacology,and the effect of isoglycyrrhizin on the target was determined by molecular docking technology and fluorescence quantitative PCR.The results showed that intragastric administration of isoglycyrrhizin could significantly reduce the number of diarrhea,delay the time of initial diarrhea and slow down intestinal peristalsis in mice.Network pharmacological studies showed that MAPK14,NCOA2,PPARG and ESR1 may be the key targets of isoglycyrrhizin in the treatment of diarrhea,isoglycyrrhizin can down regulate the expression of the above genes.Molecular docking shows that isoglycyrrhizin can bind to key proteins and is a potential good inhibitor.