紫竹盐的抗炎作用及抗肝癌活性研究

Study on anti-inflammatory effect and anti-hepatomaactivity of purple bamboo salt

为研究紫竹盐的抗炎和抗癌功效,采用炎症因子试剂盒检测粗海盐、精制海盐和紫竹盐对小鼠单核巨噬细胞RAW264.7的一氧化氮(NO),以及炎症因子肿瘤坏死因子-α(TNF-α)和白细胞介素-1β(IL-1β)含量的影响,并采用流式细胞仪测定3种盐对肝癌细胞HepG2、正常肝细胞WRL68凋亡的影响。结果表明:紫竹盐能显著抑制NO释放及降低TNF-α、IL-1β的分泌,具有较好的抗炎活性;在0.6%质量分数下,紫竹盐对肝癌细胞的凋亡率高达66.56%±5.08%,高于紫杉醇(41.16%±3.68%)、粗海盐(31.02%±4.18%)和精制海盐(29.32%±5.13%);在相同质量分数下,紫竹盐对正常肝细胞的凋亡率为8.56%±0.41%,低于紫杉醇(23.89%±2.28%)、粗海盐(21.45%±0.47%)和精制海盐(16.82%±1.49%)。紫竹盐的抗炎和抗肝癌活性研究,可为后续紫竹盐产品的开发提供理论依据。

In order to explore the effect of purple bamboo salt on anti-inflammatory and anticancer effects,the inflammatory factor kit was employed to detect the impacts of crude sea salt,refined sea salt and purple bamboo salt on the contents of nitric oxide(NO),inflammatory cytokines TNF-αand IL-1βof mononuclear macrophage RAW264.7 in mice.Then the flow cytometer was utilized to measure the impacts of those three kinds of salts on apoptosis of the hepatoma cells HepG2 and the normal hepatocytes WRL68.The results show that purple bamboo salt boasts fairly good anti-inflammatory activity by significantly augmenting the inhibition of NO release and reducing the secretion of TNF-αand IL-1β;when the mass fraction of salt being 0.6%,the apoptosis rate of hepatoma cells induced by purple bamboo salt reaches up to 66.56%±5.08%,higher than that by paclitaxel(41.16%±3.68%),crude sea salt(31.02%±4.18%)and refined sea salt(29.32%±5.13%);at the same mass fraction,the apoptosis rate of normal hepatocytes induced by purple bamboo salt is 8.56%±0.41%,much lower than that by paclitaxel(23.89%±2.28%),crude sea salt(21.45%±0.47%)and refined sea salt(16.82%±1.49%).The anti-inflammatory and anti-hepatoma activities of purple bamboo salt could provide a theoretical basis for the subsequent development of purple bamboo salt products.