舒肝颗粒对肝郁气滞型乳腺增生症小鼠的治疗作用及机制研究

Effect of Shugan Granule on mice with mammary gland hyperplasia caused by liver qi stagnation

目的探讨舒肝颗粒对肝郁气滞型乳腺增生症小鼠的治疗效果及作用机制。方法将小鼠分为空白组、模型组、中药组、西药组4组,空白组不干预,其他3组给予慢性复合应激10周。实验第11~14周,空白组、模型组给予蒸馏水灌胃,中药组、西药组分别予舒肝颗粒、他莫昔芬灌胃。于造模前、实验第10周、第14周后分别观察并记录小鼠生物表征变化。第14周处死小鼠,检测神经递质及血清激素,观察小鼠乳腺及其周围皮肤病理。结果实验第10周造模结束后,除空白组外,其余3组均出现倦怠、脱毛、体质量减轻、不动时间延长及不同程度的乳腺增生改变。中药组与模型组、西药组比较,乳腺增生程度降低,下丘脑神经递质NE和5-HT分泌被抑制,血清激素CRH和ACTH升高。结论中药舒肝颗粒能通过调节神经内分泌系统,有效减轻肝郁气滞型乳腺增生症小鼠乳腺增生程度。

Objective To observe the therapeutic effect and action mechanism of Shugan Granule on mice with mammary gland hyperplasia caused by liver qi stagnation, and explore its neuroendocrinology regulation mechanism for this disease. Methods Female mice were divided into blank group, model group, traditional Chinese medicine group and western medicine group. In the experiment, the blank group had no intervention, the other three groups were given chronic compound stress for 10 weeks. From the 11 th to 14 th week, the blank group and model group were given distilled water by gavage, while the traditional Chinese medicine group and western medicine group were given Shugan Granule and tamoxifen by gavage respectively. The biological characteristics of mice were observed and recorded after 0, 10 and 14 weeks. At the end of the experiment, the mice were killed, and the hypothalamus was stripped to detect neurotransmitters;the blood was taken to detect serum hormones;the mammary gland and its surrounding skin were taken under aseptic conditions for pathological observation. Results At the end of modeling, except the blank group, the other three groups of mice showed a series of desperate behavioral changes, such as fatigue, hair removal, weight loss, immobility time growth and so on. Compared with the other groups, hyperplasia of mammary glands was decreased, the secretion of hypothalamic neurotransmitters NE and 5-HT was inhibited, and the serum hormones CRH and ACTH were increased in the Chinese medicine group. Conclusion Shugan Granule can regulate the neuroendocrine system and reduce the degree of mammary gland hyperplasia in mice effectively.