姜味草中齐敦果酸和熊果酸的含量测定

Content Determination of Oleanolic Acid and Ursolic Acid in Micromeria Biflora Benth

目的:建立高效液相色谱法测定姜味草中齐敦果酸和熊果酸含量的方法。方法:采用高效液相色谱法测定姜味草中齐敦果酸和熊果酸的含量,色谱柱为Thermo Acclaim C_(18)(250 mm×4.6 mm, 5μm),流动相为甲醇-水-冰醋酸-三乙胺(85∶15∶0.03∶0.06),检测波长为210 nm,流速为0.8 mL·min^(-1),柱温为15℃。结果:齐敦果酸和熊果酸进样量在0.20~2.46μg (R=0.9997)和0.49~6.15μg (R=0.9999)范围内与峰面积呈良好的线性关系,精密度、重复性、稳定性实验的RSD <2%,平均加样回收率分别为98.4%和98.3%, RSD分别为2.3%和2.7%。结论:本研究建立同时测定姜味草中齐敦果酸和熊果酸含量的方法,其结果准确可靠,且具有分离度好、灵敏度高、准确性高、供试品制备简单等优点,可为姜味草质量评价及标准提升提供良好的方法和依据。

Objective To HPLC method was established for the determination of Oleanolic acid and ursolic acid in Micromeria biflora Benth.Methods The contents of Oleanolic acid and Ursolic acid in Micromeria biflora Benth were determined by HPLC,The chromatographic column is Thermo Acclaim C_(18)(250 mm×4.6 mm,5μm),The mobile phase was methanol-water-Glacial acetic acid-Triethylamine(85∶15∶0.03∶0.06),The UV detection wavelength was 210 nm,The flow rate was 0.8 mL/min,The column temperature was 15℃.Results There was a goog linear relationship between the volume of oleanolic acid and ursolic acid in the range of 0.20~2.46μg(R=0.9997)and 0.49~6.15μg(R=0.9999),The RSDs of precision,stability,repeatability tests were less than 2%,The average recoveries were 98.4%(RSD=2.3%)and 98.3%(RSD=2.7%).Conclusion A method for simultaneous determination of oleanolic acid and ursolic acid in Micromeria biflora Benth,The results are accurate and reliable,with good separation,high sensitivity,high accuracy and simple preparation,It can provide a good method and basis for quality evaluation and standard promotion of Micromeria biflora Benth.