5XFAD转基因小鼠的肠道菌群变化分析

Analysis of changes in gut microbiota of 5XFAD transgenic mice

目的:探究5XFAD阿尔茨海默病(AD)转基因小鼠相比野生型(WT)小鼠的肠道菌群变化以及性别因素的影响。方法:采集不同性别5XFAD小鼠和WT小鼠的粪便,使用高通量测序方法对粪便基因组DNA进行16S rRNA测序。再通过nf-core和基于R语言平台的生信分析手段对测序数据进行差异分析和功能预测。结果:与WT小鼠相比,在菌群构成的门水平上,5XFAD转基因小鼠的ε-变形菌门(Epsilonbacteraeota)和变形菌门(Proteobacteria)丰富度普遍较低。在属水平上,5XFAD转基因小鼠的另枝菌属(Alistipes)和乳杆菌属(Lactobacillus)丰富度明显偏高,螺杆菌属(Helicobacter)的丰富度偏低。5XFAD小鼠与WT小鼠的菌群功能也存在差异。此外,菌群的结构和功能在不同性别小鼠中也显示出差异。结论:5XFAD转基因小鼠的肠道菌群与WT小鼠存在结构和功能差异,且这种差异受性别因素影响。

Objective:To explore the gut microbiota changes of 5XFAD transgenic mice of Alzheimer's disease(AD)model compared with wild-type(WT)mice and the impacts of gender difference.Methods:The feces of different gender 5XFAD and WT mice were collected,and the bacterial 16S rRNA of fecal genomic DNA was amplified by PCR and sequenced by using high-throughput sequencing methods.Then,the differential and functional analysis of the sequencing data are carried out through nf-core and the bioinformation analysis method based on the R language platform.Results:In the composition analysis,compared to WT mice,5XFAD transgenic mice had lower abundance of Epsilonbacteraeota and Proteobacteria at the phylum level.At genus level,5XFAD transgenic mice had higher abundance of Alistipes and Lactobacillus and lower abundance of Helicobacter.In the functional analysis,compared to WT mice,5XFAD transgenic mice had influenced different metabolism pathways.Besides,the composition and function of microbiota was different in a gender specific manner.Conclusion:The gut microbiota of 5XFAD transgenic mice is different from that of WT mice,and the difference is affected by gender.