茯苓、白芷、白扁豆混合物中总多糖提取工艺优化

Optimization of extraction process of total polysaccharides from tuckahoe,angelica and Baibiandou mixture

选用茯苓、白芷、白扁豆饮片混合料(1∶1∶1),采用浸提法提取其总多糖。以总多糖得率为评价指标,考察提取次数、提取温度、提取时间、液料比对总多糖提取效果的影响,通过单因素试验与正交试验优化总多糖提取工艺。结果表明,最佳茯苓、白芷、白扁豆混合料总多糖提取工艺为提取3次、提取温度95℃、提取时间2.5 h和液料比10:1(mL:g)。在此优化条件下,总多糖得率为8.88%。以期为茯苓、白芷、白扁豆综合应用提供参考。

Total polysaccharides were extracted from tuckahoe,angelica and Baibiandou mixture(1∶1∶1)by extraction method.Taking yield of total polysaccharides as the evaluation index,the effects of extraction times,extraction temperature,extraction time,and liquid to solid ratio on the extraction effect of total polysaccharides were investigated.The results showed that the optimum extraction conditions for total polysaccharides of tuckahoe,angelica and Baibiandou mixture were as follows:extraction times 3,extraction temperature 95℃,extraction time 2.5 h,and liquid to solid ratio 10∶1(ml∶g).Under these optimum conditions,the yield of total polysaccharides was 8.88%,which could provide reference for the comprehensive utilization of tuckahoe,angelica and Baibiandou.