UPLC法测定黄黑小斑蝥中的斑蝥素

Determination of cantharidin in Mylabris cichorii Linnaeus by UPLC

建立一种超高效液相色谱法(UPLC)测定斑蝥素的含量,同时比较了野生与人工饲养黄黑小斑蝥(Mylabris cichorii Linnaeus)中斑蝥素含量差异。前处理以1%盐酸/丙酮作溶剂,超声提取斑蝥素;采用C_(18)为分析柱,流动相为乙腈-水(40∶60),紫外检测波长为230 nm,流速0.2 mL/min,柱温35℃,进样量2μL。结果表明,斑蝥素在0.02~1.00 mg/mL线性关系良好(R^(2)=0.9999);不同加标水平下平均回收率为97.9%~100.9%,RSD在1.7%~3.8%。采用该法测得野生斑蝥斑蝥素含量为3.080%,人工饲养斑蝥斑蝥素含量为1.680%,两者均高于药典中斑蝥药材的斑蝥素含量不得少于0.35%的要求。该方法准确、可靠,适用于斑蝥中斑蝥素的测定,人工饲养黄黑小斑蝥亦可用作斑蝥素的来源药材。

A method for the determination of cantharidin by ultra performance liquid chromatography(UPLC)was established,and the difference in content of cantharidin between wild and artificial feeding Mylabris cichorii Linnaeus was compared.Cantharidin was extracted with 1%hydrochloric acid/acetone solution by ultrasonic,the UPLC method was performed on C_(18) column with mobile phase consisted of acetonitrile-water(40∶60)at flow rate of 0.2 mL/min,ultraviolet detector was adopted inwavelengh of 230 nm,the column temperature was 35℃,and injection volume was 2μL.The results showed that cantharidin showed a good linear relationshipin at the range of 0.02~1.00 mg/mL(R^(2)=0.9999).The average recovery of cantharidin was in the range of 97.9%~100.9%at different spiked levels,and RSD was in the range of 1.7%~3.8%.The content of cantharidinin wild and artificial feeding Mylabris cichorii Linnaeus was 3.08%and 1.68%respectively,which were both higher than that required in Chinese Pharmacopoeia the cantharidin content in medichine material source should not be less than 0.35%.This method is accurate and reliable for the determination of cantharidin in cantharides.The artificial feeding Mylabris cichorii Linnaeus can be used as medicinal source of cantharidin.