CREB/BDNF信号通路激活在游泳提高认知能力中的作用机制

Mechanism of CREB/BDNF Signaling Pathway Activation in Improving Cognitive Ability in Swimming

目的观察有氧运动对认知的影响,探索相关分子机制。方法雄性SD大鼠适应性喂养后随机分为C、E5和E5P组。C组常规饲养不运动,E5、E5P组进行5周游泳运动,E5P组完成运动后延迟1周进行测试。Morris水迷宫测认知能力;免疫荧光法测齿状回神经发生;试剂盒测血清BDNF、MDA水平和SOD活性;免疫印迹法测海马BDNF、TrkB、CREB和p-CREB蛋白表达。结果E5、E5P组在目标象限游泳时间百分比、穿越平台次数显著高于C组(P<0.01 or P<0.05);血清BDNF、MDA水平和SOD活力组间无显著差异;E5、E5P组新生神经细胞数、海马BDNF、TrkB、p-CREB蛋白表达显著高于C组(P<0.01 or P<0.05),CREB蛋白的表达组间没有显著差异。结论5周中等强度的游泳运动通过激活CREB/BDNF信号通路,促进海马齿状回颗粒下层神经发生,进而提高认知能力。

The objective of this study was to observe the effects of 5-week aerobic exercise on the cognitive ability of rats and the continuity of the effect,and to explore its related molecular mechanisms.After adaptive feeding,male SD rats were randomly divided into control group(C),5-week exercise group(E5),and 5-week exercise group(E5P),with 8 rats in each group.The C group was routinely raised without exercise,the E5 and E5P groups were subjected to 5 weeks of moderate-intensity swimming,and the E5P group was delayed for 1 week to perform various tests after the swimming intervention.Morris water maze was used to test rat’s cognitive ability;immunofluorescence method was used to detect hippocampal dentate gyrus neurogenesis;ELISA and biochemical kits were used to detect serum BDNF,MDA levels and SOD activity.Western blot was used to detect the expression of hippocampal BDNF,TrkB,CREB and p-CREB protein.Results showed the percentage of swimming time in the target quadrant and the number of crossing platforms in the E5 and E5P groups were significantly higher than those in the C group(P<0.01 or P<0.05).There was no significant difference in serum BDNF,MDA levels and SOD activity among the three groups(P>0.05).The number of new neurons in the granular cell layer of the hippocampus in the E5 and E5P groups,the expression of hippocampal BDNF,TrkB,and p-CREB protein were significantly higher than those in the C group(P<0.01 or P<0.05),while there was no statistical difference in total CREB protein expression among the three groups(P>0.05).It could be concluded that the 5-week moderate-intensity swimming exercise could activate the CREB/BDNF signaling pathway,promote neurogenesis of hippocampal dentate gyrus,and improve the cognitive ability of rats.