云南烟草抗感PVY品种上Potyvirus属病毒种类调查

Investigation of Potyvirus Species on Resistant and Susceptible Tobacco Varieties to PVY in Yunnan Province

【目的】为云南省烟草抗PVY品种布局和病害防控等提供依据。【方法】2017—2019年在云南省5个市共13个植烟县(区)采集497个表现Potyvirus属病毒典型症状的烟草样品,并分析抗/感PVY品种上Potyvirus属病毒的种类。【结果】497个样品中Potyvirus属病毒检出率85.1%,烟草脉带花叶病毒(Tobacco vein banding mosaic virus,TVBMV)、红辣椒脉斑驳病毒(Chilli veinal mottle virus,ChiVMV)、马铃薯Y病毒(Potato virus Y,PVY)和烟草蚀纹病毒(Tobacco etch virus,TEV)的检出率分别为58.8%、23.3%、14.9%和7.8%。感PVY品种上Potyvirus属病毒检出率地区间差异较大,曲靖样品中TVBMV的检出率最高,玉溪样品中ChiVMV检出率最高,昭通样品中PVY检出率最高。在Potyvirus属病毒典型症状样品中,抗PVY品种比感PVY品种的PVY检出率低16.7%,抗PVY品种表现出良好的病害防治效果。【结论】昭阳区和澄江品种园的样品检测结果均证明,抗PVY品种的TVBMV检出率升高,是由于PVY发病率降低导致的,抗PVY品种不会导致TVBMV和ChiVMV危害上升。PVY高发病区可通过种植抗PVY品种来降低损失,种植前宜进行病原种类鉴定,以便将va品种布局在PVY为主的区域。云南主要烟区TVBMV和ChiVMV的发病率高,缺乏抗病品种,需开展抗TVBMV和ChiVMV的种质资源鉴定、发掘和创制。

【Objective】The purpose of this paper is to obtain the basis information of Potyviruses distribution and investigate Potyvirus species between resistant and susceptible tobacco varieties to PVY in field in Yunnan Province.【Method】During 2017 to 2019,497 tobacco samples with typical Potyviral symptoms were collected in 5 cities,13 tobacco planting counties(districts)in Yunnan Province and the types of Potyviruses on the main susceptible and resistant varieties to PVY were analyzed.【Result】The results showed that 85.1%of 497 tobacco samples were infected by Potyviruses in total.Furthermore,the detection rates of TVBMV,ChiVMV,PVY,and TEV were 58.8%,23.3%,14.9%and 7.8%respectively.In terms of the rank of detection rates of Potyviruses in the PVY susceptible varieties,the detection rate of TVBMV was the highest in Qujing,and for ChiVMV was in Yuxi,and for PVY was in Zhaotong.The PVY detection rate on the PVY-resistance varieties was 5.8%around and much lower than that of susceptible varieties(22.5%).PVY resistant varieties showed a good crop protection for the PVY disease.【Conclusion】The analysis showed that the resistant varieties which contained va locus dramatically reduced the yield loss in the high incidence areas of PVY,and the increase of TVBMV detection rate in PVY resistant varieties was due to the decrease of PVY incidence rate,and the resistant varieties did not increase the harm of TVBMV and ChiVMV.However,the single va resistant locus had no effect on the TVBMV and ChiVMV.Therefore,the investigation of Potyviruses species should be carried out before introduce va-containing varieties into the area.TVBMV and ChiVMV had been the emerging disease in Yunnan's main tobacco growing areas,resulting in low yield and quality loss.It is necessary to establish new method to detect these causal agents and to screen moreresistant germplasm resources for TVBMV and ChiVMV.