离子色谱法测定甘氨酸含量

Determination of glycine content by ion chromatography

基于甘氨酸的基本物化性质,以及滴定法检测甘氨酸含量的缺点,为规避滴定法检测的弊端,简化检测步骤,提高检测效率,采用离子色谱法对甘氨酸含量进行测定。采用单点校正的方法,将离子色谱法与滴定法检测同一样品的数据进行对比,以此得出实验结果。选用Shodex IC YS-50阳离子色谱柱,淋洗液浓度为3.5 mol/L的甲基磺酸超纯水溶液,流速为0.8 mL/min,检测器为非抑制电导检测器。实验结果表明,离子色谱法准确度高、重复性好、操作简单便捷。

Based on the basic physicochemical properties of glycine and the shortcomings of titration method to detect glycine content,in order to avoid the disadvantages of titration method,simplify the detection steps and improve the detection efficiency,the glycine content was determined by ion chromatography.The single point correction method was adopted,and the experimental results were obtained by comparing the data of the same sample detected by ion chromatography and titration method.Shodex IC ys-50 cation chromatographic column was selected,the eluent concentration was 3.5 mol/L of methylsulfonic acid ultrapure water solution,the flow rate was 0.8 ml/min,and the detector was a non-inhibitory conductivity detector.The experimental results show that ion chromatography has high accuracy,good repeatability,simple and convenient operation.