基于正交试验设计的三山散最佳提取工艺研究

Extraction process of Sanshan powder by orthogonal experiment

利用正交试验L_(9)(3^(4))设计,以三山散组方中的主药黄芩按黄芩苷含量为指标,对其提取工艺进行研究。结果表明,三山散提取工艺中煎煮次数的作用大于其他因素,其次是用水量、煎煮时间及浸泡时间。最佳工艺为用8倍量的水、浸泡1 h、煎煮1.5 h、煎煮3次。并进行验证试验,其RSD%为0.26%。该提取工艺稳定,批间差异小,可为后续的产业化开发提供参考。

The extraction process of Sanshan powder was studied by orthogonal test with baicalin content.The effect of decocting times was greater than other factors,followed by water consumption,decocting time and soaking time.The best process was with 8 times the amount of water,soaking for 1 h,decocting for 1.5 h,decocting for 3 times.Verification tests showed that the RSD was 0.26%.The extraction process was stable and the difference between batches was small,which laid a research foundation for the subsequent industrial development.