麻杏石甘汤加减协定方治疗新型冠状病毒肺炎作用机制的网络药理学研究

Network pharmacology study on the mechanism of maxingshigan decoction agreement formula in treating COVID-19

目的探究麻杏石甘汤加减协定方治疗新型冠状病毒肺炎(COVID-19)的作用机制。方法利用中药系统药理学分析平台(TCMSP)、中药综合数据库(TCMID)结合文献筛选协定方所含活性化学成分。利用BATMAN-TCM、OMIM、GeneCards等数据库收集协定方及COVID-19疾病靶点集。分析协定方抗COVID-19潜在靶点集作用于人体的组织器官及表达丰度。通过STRING数据库进行GO功能富集分析和KEGG通路富集分析,利用Cytoscape3.7.2构建加权模式下成分-靶点-通路网络,预测其作用机制。结果靶点集的35个靶点作用于人体肺、扁桃体、胆囊、小肠、胃、肾等48个组织器官。KEGG通路富集分析筛选得到MAPK信号通路、肿瘤坏死因子信号通路、Toll样受体信号通路、T细胞受体信号通路等炎性反应、肿瘤抑制以及免疫调节等15条信号通路。通过"成分-靶点-通路"网络的节点重要度及相关网络拓扑参数分析,确定AKT1、JUN、HRAS、TNF、IL6等为关键靶点。结论协定方通过多成分、多靶点、多途径提高机体免疫功能,降低炎症风暴产生的风险,保护人体呼吸系统、消化系统和循环系统组织器官,从而发挥对COVID-19的治疗作用。

Objective To explore the mechanism of Maxingshigan decoction agreement formula in the treatment of medicine and COVID-19.Methods The active chemical components contained in the Maxingshigan decoction agreement formula were screened by the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),Traditional Chinese Medicine Integrative database(TCMID) and literatures.BATMAN-TCM,OMIM and GeneCards databases were used to collect targets of medicines and COVID-19.Analyze the human tissues and organs which potential targets acting on and their expression abundance.The GO function enrichment analysis and KEGG pathway enrichment analysis were performed by the STRING database.Cytoscape 3.7.2 was used to construct a component-target-pathway network in a weighted mode to predict the mechanism.Results The 35 targets acted on 48 tissues and organs, including human lung, tonsil, gallbladder, small intestine, stomach, and kidney.KEGG pathway enrichment analysis screened 15 signal pathways, including inflammatory responses such as MAPK signaling pathway, tumor necrosis factor signaling pathway, Toll-like receptor signaling pathway, T cell receptor signaling pathway, and tumor suppression, immune regulation.Based on the node importance of the component-target-pathway network and the analysis of related network topology parameters, AKT1,JUN,HRAS,TNF,IL6,etc.were identified as key targets.Conclusion The agreement formula can improve the human immune function, reduce the risk of inflammatory storms, protect the human respiratory system, digestive system, and circulatory system tissues and organs through multiple components, multiple targets, and multiple pathways.thereby it exerts therapeutic effects on the COVID-19.