基于谷胱甘肽修饰金纳米粒子可视化检测镉离子残留的研究

Visual detection of cadmium ion residue based on glutathione modified gold nanoparticles

基于镉离子可以与谷胱甘肽的羧基进行螯合配位形成网状超分子的性质,引入谷胱甘肽(GSH)的巯基修饰金纳米粒子(AuNPs)的表面,采用自组装法制备谷胱甘肽-金纳米粒子(GSH-AuNPs),进而建立一种灵敏高效的可视化比色检测镉离子的方法。结果表明,随着镉离子浓度的增高,体系的颜色由酒红色变为蓝紫色;在1.25~3.00μmol/L范围内,镉离子浓度与吸光度具有良好的线性关系,检测限为80 nmol/L;反应体系对4种常见金属离子和5种常见氨基酸具有明显的抗干扰性能。该谷胱甘肽-金纳米可视化比色检测镉离子体系具有良好的选择性和灵敏性。

Because cadmium ions can chelate and coordinate with carboxyl group of glutathione(GSH) to form a network supramolecule, a sensitive and efficient visualizing colorimetric detection method for cadmium ions is established through using sulfhydryl group of GSH to modify the surface of gold nanoparticles(AuNPs) and then preparing GSH-AuNPs glutathione via self-assembly method.Application results of the method show that with the increasing cadmium ion concentration, the color of the system changes from wine red to blue-purple;the concentration of cadmium ions has a good linear relationship with absorbance in the range of 1.25-3.00 μmol·L;,with a detection limit of 80 nmol·L;.The reaction system has obvious anti-interference performance against 4 kinds of common metal ions and 5 kinds of common amino acids.The presented GSH-AuNPs visualized colorimetric detection system for cadmium ions exhibits good selectivity and sensitivity.