高选择性亚硝酰氢荧光探针的合成及生物应用

Synthesis and biological application of fluorescent probe with high selectivity for nitrosyl hydrogen

以6-苯并-d-噻唑-2-萘酚为荧光母体和二苯基膦苯甲酸酯为HNO的特异性识别基团,设计合成了一种荧光增强型探针(BNB-HNO),该探针对HNO具有较好的选择性、抗干扰性、高灵敏性(LOD=54.6 nmol/L)以及响应迅速(反应时间<10 min)等优点;另外,BNB-HNO可以直接用于血清样品中HNO的定量检测和斑马鱼中外源性HNO的成像研究。证明BNB-HNO具有用于生物体系中HNO检测的潜力,是生物系统中HNO的理想"追踪器"。

A turn-on fluoresce-enhanced probe(BNB-HNO) for nitrosyl hydrogen(HNO) is designed and synthesized with 6-benzo-d-thiazole-2-naphthol as the fluorescent matrix and diphenyl phosphinobenzoate as the specific recognition group of HNO.This probe presents good selectivity, strong anti-interference, high sensitivity(LOD=54.6 nmol·L;) and rapid response(less than 10 min).Moreover, BNB-HNO can be directly used for the quantitative detection of HNO in serum samples, and successfully applied to imaging of exogenous HNO in zebrafish.It is proven that BNB-HNO has the potential for HNO detection in biological system and is an ideal “tracker” for HNO in biological system.