丹参-葛根药对中7种成分的含量测定及其配伍影响考察

Content Determination and Compatibility of 7 Components in Drug Pair Salvia Miltiorrhiza and Radix Puerariae

目的:建立HPLC法同时测定丹参-葛根药对中7种成分(葛根素、大豆苷、大豆苷元、丹酚酸B、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_(A))的含量,考察药对不同配比情况下7种成分溶出率的变化。方法:采用Diamonsil(Ⅱ)C_(18)ODS(250 mm×4.6 mm, 5μm)色谱柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱;检测波长为250 nm;流速:1.0 ml·min^(-1);柱温:30℃;进样量:10μl。结果:7种成分在一定质量范围内与峰面积的线性关系良好(r≥0.999 3),平均加样回收率为98.3%~99.2%,RSD≤1.0%(n=6)。按不同比例配伍(7∶3、2∶1、1∶1、4∶3、3∶4、1∶2)的药对,7种成分溶出率均低于单味药材提取液,但配伍比例为1∶1时7种成分溶出率高于其他配伍比例。结论:所建立的含量测定方法简便、准确、重复性好。丹参-葛根药对1∶1配伍最有利于有效成分溶出。

Objective: To develop an HPLC method for the simultaneous determination of 7 components(puerarin, daidzin, daidzein, salvianolic acid B, cryptotanshinone, tanshinone I and tanshinoneⅡ_(A)) in drug pair Salvia miltiorrhiza Bunge-Radix Puerariae, and to study the variations in dissolution rates of 7 components in different proportions of drug pairs.Methods: A Diamonsil(Ⅱ)C_(18)ODS(250 mm×4.6 mm, 5 μm) chromatographic column was used;the mobile phase was acetonitrile-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 ml·min^(-1)and the detection wavelength was 250 nm, the column temperature was 30 ℃, and the sample size was 10 μl. Results: The liner relationships of seven effective components were favorable, the mean recoveries were 98.3%-99.2%, and the RSDs were no more than 1.0%(n=6). Compared with those in the extracts of single Salvia miltiorrhiza Bunge and Radix Puerariae, the 7 components decreased in various degrees in 6 various combination of Salvia miltiorrhiza Bunge-Radix Puerariae(7∶3, 2∶1, 1∶1, 4∶3, 3∶4, 1∶2), the dissolution rate of the 7 components was the highest when the ratio of Salvia miltiorrhiza Bunge-Radix Puerariae was 1∶1, suggesting the optimal compatibility proportion was 1∶1. Conclusion: The established method is simple, accurate and reproducible, and drug pair Salvia miltiorrhiza Bunge-Radix Puerariae at the proportion of 1∶1 has an obvious promoting effect on the dissolution of the components.