石菖蒲和茴香菖蒲的生药学及GC-MS比较分析研究

Histomorphology and GC-MS analysis of Acorus tatarinowii and Acorus gramineus Soland

目的对石菖蒲和茴香菖蒲的性状、显微特征、挥发油主要成分及含量等方面进行鉴别研究,以确保临床用药的安全性和准确性。方法采用了性状、显微鉴别和水蒸气提取方法,利用GC-MS法对挥发油中的成分进行了分析,并采用峰面积归一化法计算各成分相对百分含量。结果明确了石菖蒲、茴香菖蒲的性状、显微鉴别特征。对两者提取的挥发油的化学成分进行了定性分析,所鉴定的成分分别占总流出峰面积的99.71%和99.99%。结论根据性状和组织显微特征比较,以及对挥发油的GC-MS分析,可以准确、快捷鉴别石菖蒲与茴香菖蒲,以保证临床用药的准确性和安全性,为促进二者的开发利用提供实验数据。

Objective The characters,microscopic characteristics,main components and contents of volatile oil of Acorus tatarinowii and Acorus gramineus Soland were studied to ensure the safety and accuracy of clinical medication.Methods Characterization,microscopic identification and water vapor extraction methods were used.The constituents of volatile oil were analyzed byusing the method of GC-MS,and the relative percentage of each component was calculated by peak area normalization method.Results The characteristics and microscopic identification of Acorus tatarinowii and Acorus gramineus Soland were clarified.The chemical components of volatile oil extracted from the two were analyzed qualitatively.The identified components accounted for 99.71%and 99.99%of the total outflow peak area respectively.Conclusion According to the comparison of morphological and histological characteristics and GC-MS analysis of volatile oil,Acorus tatarinowii and Acorus gramineus Soland can be accurately and quickly identified,To ensure the accuracy and safety of clinical medication,and to provide experimental data for promoting the development and utilization of both.