基于肠道菌群的黑果腺肋花楸多酚降压作用初探

Preliminary Studies on the Antihypertensive Effect of Polyphenols from Aronia Melanocarpa Based on Intestinal Flora

以黑果腺肋花楸多酚(AMP)为原料,探究其对高盐、高糖饮食所致高血压大鼠模型的血压调节及肠道菌群的影响。对Waster大鼠进行20%果糖饮水和5%NaCl喂养试验21 d,结束后取其粪便进行肠道菌群丰富度分析、菌群群落分析、Heatmap分析、COG数据库对比分析。结果显示,AMP对高血压的降压效果显著,且降压效果不是单纯的随浓度的升高而升高,其中AMP中剂量组(质量浓度为1.4 mg/kg,每日灌胃剂量7.5 mL/kg)的降压效果最好,降压达11.6%。AMP不仅能调节肠道菌群微环境,还对高血压大鼠的肠道功能有较好的改善作用,有利于提高降压效果。与常规降压药相比,AMP对肠道菌群影响小,且可增加一些有益菌丰富度,如乳酸菌较正常组提高59.2%。本研究结果为AMP作为调节高血压的功能性因子,制备相关功能性食品提供理论依据。

In this paper,the effects of aronia melanocarpa polyphenols(AMP)from aronia melanocarpa fructus on blood pressure regulation and intestinal flora in rats with hypertension induced by high-salt and high-sugar diet were investigated.After Waster rats were fed with 20%fructose water and 5%NaCl for 21 days,their feces were collected for intestinal flora richness analysis,flora analysis,Heatmap analysis and comparative analysis of the COG database.The results showed that AMP had a positive effect on hypertension,and the antihypertensive effect of polyphenols does not simply increase with the increase of concentration.And medium dose AMP(The concentration is 1.4 mg/kg,and the daily intragastric dose is 7.5 mL/kg)had the best antihypertensive effect,the antihypertensive effect reached 11.6%.In addition,AMP can not only regulate the intestinal microenvironment,but also improve the intestinal function of hypertension rats,which is beneficial to improve the therapeutic effect of hypertension diseases.Compared with conventional antihypertensive drugs,AMP had less effect on intestinal flora,and the richness of some beneficial bacteria was increased,such as Lactobacillaceae increased by 59.2%respectively compared with the normal group.Therefore,this study provides theoretical basis for the use of AMP as functional factors in the regulation of hypertension and the preparation of relevant functional foods.