膳食纤维对肠道菌群紊乱及脂代谢异常的修复作用

Reparative Action of Dietary Fiber on Intestinal Flora Disorder and Lipid Metabolish

目的:探究膳食纤维对肠道菌群紊乱及其引起的脂代谢异常的修复作用。方法:通过头孢曲松钠灌胃处理建立小鼠肠道菌群紊乱模型,采用菊粉进行干预。通过宏基因组学测定小鼠肠道菌群组成;采用ELISA试剂盒测定小鼠血脂指标;采用气相色谱仪测定小鼠粪便中短链脂肪酸含量及血清、肝脏中的脂肪酸谱;采用液-质谱联用测定小鼠粪便胆汁酸组成及含量。结果:采用菊粉进行干预,能促进小鼠肠道菌群结构及血脂的修复;显著提高粪便中短链脂肪酸含量;显著修复抗生素处理引起的血清、肝脏中脂肪酸组成的变化。胆汁酸分析结果表明,菊粉能部分修复菌群失调导致的胆汁酸含量变化。结论:菊粉能通过胆汁酸-FXR信号通路调节肠道菌群紊乱及脂代谢异常。

Objective:This work was to explore the repaired position of dietary fiber on lipid metabolism shifts caused by gut microbiome dysbiosis.Methods:We built the animal model for the study with gut microbiome dysbiosis administrated by i.g.with CRO and intervened by inulin.Gut microbiome was analyzed by high throughput sequencing and bioinformatics analyses of bacteria.The composition of fatty acids and short chain fatty acids(SCFAs)were determined by gas chromatography.Blood lipids and bile acids were assayed by kit and UPLC-MS/MS,respectively.Results:Inulin improved the profiles of gut microbiome and repaired the lipids metabolism shifts from gut microbiome dysbiosis.SCFAs were remarkably high after treatment by inulin.Besides,inulin regulated fatty acids metabolism and partially repaired bile acid profiles.Conclusion:It was recommended that inulin repaired lipid metabolism shifts from gut microbiome dysbiosis via bile acids-FXR pathway.