碳量子点/纳米金@碳纳米管修饰电极同时检测鸟嘌呤和腺嘌呤

Simultaneous voltammetry detection of guanine and adenine in human serum with a CQDs/AuNPs @MWCNTs modified glassy carbon electrode

制备了一种碳量子点(CQDs)/金纳米颗粒(AuNPs)@羟基化多壁碳纳米管(MWCNT-OHs)复合膜修饰电极用于鸟嘌呤(GA)和腺嘌呤(AE)的同时检测。与裸电极和其它修饰电极相比,复合膜修饰电极能显著提高GA和AE的氧化峰电流及峰电位差,能够对GA和AE同时高灵敏检测。研究了GA和AE在复合膜修饰电极上的电化学行为,结果表明,在0.2 mol/L PBS(pH 7.0)中,GA和AE的氧化峰电流与浓度分别在1~200μmol/L和2~80μmol/L范围内呈良好的线性关系,检测限分别为0.9和1.8μmol/L。将该修饰电极用于人体血清样品中GA和AE的同时电化学检测,加标回收率在90.4%~107.4%之间,证明了该修饰电极在生物样品分析领域的应用潜力。

A modified glass carbon electrode(GCE) based on carbon quantum dots(CQDs) and gold nanoparticles(AuNPs)@hydroxylated multi-wall carbon nanotubes(MWCNT-OHs) composite was fabricated and then employed to the selective and simultaneous determination of guanine and adenine. Compared with the other electrodes, the as prepared CQDs/AuNPs@MWCNTs-OH modified electrode gave the highest peaks current and best peaks potential resolution. The electrochemical behaviors of guanine and adenine on the electrode were carefully investigated, and the results showed that, under the optimal condition, the oxidation peak currents concentrations of the analytes were linear with their concentrations in the range of 1-200 μmol/L and 2.0-80 μmol/L, with the detection limits of 0.9 and 1.8 μmol/L for guanine and adenine, respectively. The modified electrode was used to simultaneous electrochemical analysis of GA and AE in human serum samples. The recoveries were between 90.4%-107.4%, which proved that the modified electrode was effective in biological samples.