甲氨蝶呤磷脂复合物纳米粒对乳腺癌MCF-7细胞的体内外抗肿瘤作用

Antitumor Effect of MTX-PC Complex-Nanoparticles on Breast Cancer MCF-7 Cell in vitro and in vivo

研究甲氨蝶呤磷脂复合物纳米粒(MTX-PC NPs)对乳腺癌MCF-7细胞的抗肿瘤作用。选择乳腺癌MCF-7细胞为体外模型,采用MTT比色分析法,观察体外乳腺癌细胞的生长和增殖抑制情况,评价MTX-PC NPs对MCF-7细胞的毒性作用;采用激光共聚焦荧光显微镜技术和流式细胞术观察其对MCF-7细胞摄取药物的影响;采用小鼠MCF-7细胞实体瘤模型进行体内抑瘤实验,观察移植瘤动物的生长情况,测定肿瘤大小。结果表明,MTX-PC NPs能抑制MCF-7细胞的生长增殖,且随药物浓度的升高而增强;激光共聚焦荧光照片显示载药纳米粒能有效地被MCF-7细胞摄取;对于FA(叶酸)受体过度表达的MCF-7细胞有高亲和识别,而对正常NIH-3T3细胞摄取较少;与MTX注射剂相比,相同药物浓度下,MTX-PC NPs组的小鼠MCF-7实体瘤生长更缓慢,表明抑制作用明显。说明甲氨蝶呤磷脂复合物纳米粒可能对乳腺癌有更优良的治疗作用。

To study the antitumor effect of MTX-PC Complex-Nanoparticles(MTX-PC NPs)on breast cancer MCF-7 cell in vitro and in vivo,the breast cancer MCF-7 cell in vitro were selected,the cell growth and viability inhibited by MTX-PC NPs were observed and the antitumor cytotoxicity was evaluated by MTT assay.Cellular uptake of MTX was observed by Confocal fluorescence microscopy(CLSM)and Flow cytometry(FCM).MCF-7 cell was subcutaneously injected into mice and MTX-PC NPs was administered to the tumor-bearing mice to observe the growth of mice and to measure the growth of tumor.MCF-7 cell growth and viability were inhibited by MTX-PC NPs,the inhibition was enhanced with increasing concentrations.CLSM photos indicated that MTX-PCNPs enhance the intracellular uptake of MTX in MCF-7 cells.MCF-7 cell with overexpressing FA receptors are highly recognition,while normal NIH-3 T3 cells were less abundant.Compared with MTX injection,MTX-PC NPs group of tumors grew more slowly.It has shown that MTXPC NPs had a more pronounced inhibitory effect on the growth of MCF-7 tumors in mice at the same drug concentration.So MTX-PC NPs maybe had a better therapeutic effect on human breast cancer.