杂交水稻骨干亲本鉴定标记的开发及应用

Development and Application of Identification Markers for Main Parental Lines of Hybrid Rice

随着现代杂交稻育种的发展,利用表型进行品种特异性鉴定、品种真实性鉴定以及种子纯度测试的方法表现出诸多局限性,已经不再适合现代育种的进程。本研究利用水稻基因组插入缺失(insertion-deletion,InDel)位点开发了9个In Del标记用于鉴定生产中常用的29份杂交稻骨干亲本。利用标记电泳图谱做了供试材料的聚类分析,在遗传相似系数0.55左右可将所有供试材料分为5个类群,另外根据不同的亲缘关系和来源地区,又可以将供试材料分为9个亚群。最后利用品种鉴别标记和生长测试两种方法对一份杂交稻种子‘广两优476’纯度进行了检测,结果表明两者基本一致。因此,分子标记可以有效应用于现代育种的各个环节,从而加快新品种选育。本研究结果将为水稻育种、新品种审定、品种鉴定以及种子生产提供有益的帮助。

As the development of the modern hybrid rice breeding,many disadvantages behaved in variety identification,variety authenticity identification and seed genetic purity test based on phenotype,which was not suitable for the modern breeding.Insertion-deletion(InDel)loci in rice genome sequence were used to develop 9 InDel markers to identify 29 hybrid rice backbone parental lines which were widely used in rice production.Cluster analysis of 29 parental lines were conducted using electrophoresis of those InDel markers,and they were clustered into five groups with similarity coefficients of about 0.55.In addition,according to relatedness and original region these lines were clustered into nine subgroups.Genetic purity was conducted for a hybrid rice’Guangliangyou476’using InDel marker and growth-out test methods,and similar results were generated.So molecular marker could to be put used into each stage of the modern breeding to accelerate breeding new variety.This research will be helpful for rice breeding,new cultivar registration and seed production.