摘要
本文对长叶紫珠(Callicarpa longifolia)进行化学成分研究。运用硅胶、Sephadex LH-20、ODS、MCI等柱层析并结合半制备型HPLC、重结晶等手段对长叶紫珠的甲醇提取物进行分离纯化;通过显色反应、理化性质、核磁共振波谱技术、质谱法等对所分离得到的化合物进行结构鉴定,从长叶紫珠中分离得到10个化合物,分别为18-hydroxyferruginol(1)、dehydroabietanol(2)、neolupenol(3)、kaempferol-3,4′,7-O-trimethylether(4)、artemein(5)、5-羟基-7,8,3′,4′-四甲氧基黄酮(6)、penduleti(7)、毛蕊花糖苷(8)、邻苯二甲酸二(2-乙基己基)酯(9)、邻苯二甲酸二丁酯(10)。十个化合物均为首次从长叶紫珠植物中分离得到。运用Griess法测定化合物1~5、8~10对LPS诱导的RAW 264.7细胞模型NO释放量的影响研究其抗炎活性。结果显示,化合物1、2、4、10具有一定的抑制NO生成作用,其IC_(50)值分别为25.2、34.6、109.6、104.8μmol/L。
To study the chemical constituents of Callicarpa longifolia.The methanol extract of C.longifolia was isolated and purified by silicon,Sephadex LH-20,ODS,MCI column chromatographies,semi-preparative HPLC and recrystallization method.The structures of the compounds were identified by color reaction,physicochemical properties,nuclear magnetic resonance spectroscopy and mass spectrometry.Ten compounds,18-hydroxyferruginol(1),dehydroabietanol(2),neolupenol(3),kaempferol-3,4′,7-O-trimethylether(4),artemiin(5),5-hydroxy-7,8,3′,4′-tetramethoxyflavone(6),penduleti(7),verbascoside(8),di-(2-ethylhexyl)-phthalate(9),and dibutyl phthalate(10)were isolated from C.longifolia for the first time.The anti-inflammatory effect of compounds 1-5 and 8-10 was tested on lipopolysaccharide-stimulated RAW 264.7 macrophages.The results showed that compounds 1,2,4 and 10 had significant inhibitory effects on NO production and their IC_(50) values were 25.2,34.6,109.6 and 104.8μmol/L respectively.
作者
赵璐
徐凡
易侨祺
张桃莉
王红刚
ZHAO Lu;XU Fan;YI Qiao-qi;ZHANG Tao-li;WANG Hong-gang(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《天然产物研究与开发》
CAS
CSCD
2021年第12期2029-2034,共6页
Natural Product Research and Development
基金
广东省省级科技计划(2016A070712021)。
关键词
长叶紫珠
紫珠属
化学成分
抗炎
Callicarpa longifolia
Callicarpa
chemical constituents
anti-inflammation