HPLC-MS/MS检测代餐粉中6种减肥及4种降糖的非法添加物

Detection of 6 kinds of Weight-reducing and 4 kinds of Hypoglycemic addition in meal replacement powder by HPLC-MS/MS

应用高效液相色谱-串联质谱(HPLC-MS/MS)建立了同时检测代餐粉中6种减肥类(安非他明、芬特明、氯卡色林、氟苯丙胺、西布曲明、酚酞)及4种降血糖类(二甲双胍、格列吡嗪、瑞格列奈、格列本脲)非法添加物质的检测方法。代餐粉样品用甲醇提取,经SB C18色谱柱(Agilent,2.1 mm×150 mm,3.5μm)分离,应用电喷雾离子正模式(ESI+)进行检测。结果表明,10种非法添加物定量限为1.0 ng·g^(-1),线性相关性较好,相关系数均不低于0.9996。平均添加回收率在84.6%~106.0%之间,相对标准偏差在0.93%-5.84%之间。实验通过对色谱条件、前处理方法及质谱参数进行优化,建立了代餐粉中10种减肥及降糖非法添加物检测方法,方法检出限低、操作简单。

The application of the high performance liquid chromatography-tandem mass spectrometry(HPLCMS/MS)method.Establish simultaneous testing of 6 types of weight-reducing n health care products(Amphetamine、phentermine、Lorcaserina、fenfluramine、Sibutramine、Phenolphthalein)and 4 Hypoglycemic(Metformin、Glipizide、Repaglinide、Glibenclamide)detection methods for illegal addition of substances.Samples of Internet-famous Meal replacement powder are extracted with methanol,Separated by SB C18 columns(Agilent,2.1 mm×150 mm,3.5 m),The electric spray ion positive mode(ESI+)is used for detection.The results show that,the The quantitative limit for 10 illegal additives is 0.5 ng·g^(-1),linear correlation is good,The correlation coefficients are not less than 0.9996,The average add-on recovery rate is between 84.6%-106.0%.The relative standard deviation is between 0.93%-5.84%.The experiment is optimized by chromatography conditions,pretreatment methods and mass spectrometry parameters,An ideal test method for the subject to be measured is established,which is simple,and the results are reliable.