Ti-Zr-Cu合金的抗菌性能和体外生物相容性

Antibacterial Property and in vitro Biocompatibility of a Ti-Zr-Cu Alloy

采用平板共培养法测定Ti-Zr-Cu合金表面的菌落数,通过CCK8细胞增殖检测、鬼笔环肽细胞染色观察细胞核和细胞骨架形貌、细胞凋亡和黏附能力测定,研究了Ti-Zr-Cu合金的抗菌性能和体外生物相容性。结果表明,空白样品和Ti-Zr合金表面上的菌落密集,而Ti-Zr-Cu合金表面上的菌落数极少。Ti-Zr-Cu合金对金黄色葡萄球菌和大肠杆菌的抗菌率分别达到98.28%和97.67%,表现出优异的抗菌性能。MC3T3-E1在Ti-Zr-Cu合金表面培养1、4、7 d的相对增值率分别为168.8%、109.8%和106.5%,均高于100%,表明这种合金没有细胞毒性。细胞在Ti-Zr-Cu合金表面上的附着和铺展良好,有利于贴壁细胞在其表面黏附和进一步增值,表明Ti-Zr-Cu合金具有良好的生物相容性。Ti-Zr-Cu合金对细胞凋亡没有不良影响。从Ti-Zr-Cu合金表面细胞的SEM照片可见细胞的粘附正常,也表明其具有良好的生物相容性。

The antibacterial property and in vitro biocompatibility of a novel Ti-Zr-Cu alloy were investigated.Meanwhile,the number of bacterial colonies on the surface of Ti-Zr-Cu alloy was determined by plate co-culture method,and the morphology of nuclear and cytoskeleton,cell apoptosis and adhesion ability on Ti-Zr-Cu alloy were assessed by means of CCK8 cell proliferation detection and phyllopeptide cell staining observation.The results show that the density of bacterial colonies on the blank sample and Ti-Zr alloy surface was very high,while the number of bacterial colonies on the Ti-Zr-Cu alloy surface was very low.The antibacterial rate of Ti-Zr-Cu alloy against Staphylococcus aureus and Escherichia coli reached 98.28%and 97.67%,respectively,showing excellent antibacterial properties.The relative productivity of MC3T3-E1 cultured on Ti-Zr-Cu alloy surface for 1,4 and 7 days were 168.8%,109.8%and 106.5%,respectively,which were all higher than 100%,indicating that this alloy had no cytotoxicity.The adhesion and spread of cells on the surface of Ti-Zr-Cu alloy are good,which is conducive to the adhesion and further growth of adherent cells on the surface of Ti-Zr-Cu alloy,indicating that Ti-Zr-Cu alloy has good biocompatibility.Ti-Zr-Cu alloy had no adverse effect on cell apoptosis.The SEM images of the cells on the surface of Ti-Zr-Cu alloy showed that the adhesion of the cells was normal,which also indicated that the cells had good biocompatibility.