辅因子工程在微生物代谢中的应用进展

Application Progress of Cofactor Engineering in the Microbial Metabolism

辅因子参与活细胞中几乎所有酶促反应,其中NAD(P)H/NAD(P)+在微生物代谢中起重要作用。NAD(P)H/NAD(P)+不稳定会导致微生物胞内氧化还原失衡,使细胞生理特性发生改变。通过辅因子调控策略可促进氧化还原平衡,使碳代谢通量能够最大限度流向目标代谢产物。面对日益突出的能源消耗和环境问题,促使辅因子工程广泛应用于食品工业生产高附加值产品。本文总结近年来辅因子在微生物代谢与目标产物合成中的影响,以期为食品工业的高效生产提供参考。

Cofactors are involved in almost all enzymatic reactions in living cells,where the ratios of NAD(P)H/NAD(P)+plays a significant role in microbial metabolism.The instability of NAD(P)H/NAD(P)+can cause intracellular redox imbalance and change cell physiological characteristics.The cofactor regulation strategy promotes redox balance,and carbon flux can flow to the target product to the maximum extent.Facing the increasingly prominent energy consumption and environmental issues,cofactor engineering has widely used to produce high-value products in the food industry.This work briefly reviews the role of various cofactors in microbial metabolism,and summarizes recent advance on the development and application of cofactor engineering in the food industry,providing a reference for the efficient production in food industry.