QuECHERS-HPLC测定大白菜中阿维菌素残留量

QuECHERS-HPLC Determination of Abamectin Residues in Chinese Cabbage

建立大白菜中阿维菌素残留量的准确、快速检测方法。采用QuECHERS-高效液相紫外法测定大白菜中的阿维菌素,用乙腈和萃取试剂(MgSO_(4)、NaCl、柠檬酸盐混合物)提取试样,漩涡振荡、离心;再通过PSA、GCB、MgSO_(4)净化,漩涡振荡、离心;最后收集经0.22μm有机滤膜过滤的滤液上机检测。在阿维菌素质量浓度0.1~10.0mg/L范围内,线性关系较好,相关系数R^(2)=0.99998。0.2 mg/L、2.0 mg/L、4.0 mg/L、10.0 mg/L 4组阿维菌素质量浓度测定的重复性好,RSD在0.03%~0.28%之间。空白样品试样在0.2mg/L、0.4mg/L、0.5mg/L、1.0mg/L 4个浓度添加水平的回收率为81.35%~100.67%。该方法操作简单、方便快捷、灵敏度高、重复性好、稳定性好、结果满意,适用于大白菜中阿维菌素的准确、快速检测;高效液相色谱仪配紫外检测器的普及率高,该方法具有较强的操作性和基层推广意义。

To establish an accurate and rapid method for the determination of abamectin residue in Chinese cabbage.The QuECHERS-high performance liquid(HPLC)-UV method was used to determine the abamectin in Chinese cabbage.The sample was extracted with acetonitrile and extraction reagent(the mixture of MgSO_(4),NaCl and citrate),vortexed,centrifuged;and then the sample was purified by PSA,GCB and MgSO_(4),vortexed,centrifuged;and finally collect the filtrate filtered through a 0.22μm organic filter membrane for machine testing.In the range of abamectin mass concentration 0.1~10.0 mg/L,the linear relationship was good,and the correlation coefficient R^(2)=0.99998.The reproducibility of abamectin mass concentration in the 4 groups of 0.2 mg/L,2.0 mg/L,4.0 mg/L and 10.0 mg/L was good,and the RSD is between 0.03%and 0.28%.The recovery rate of blank samples were 81.35%~100.67%at the four supplemental levels of 0.2 mg/L,0.4 mg/L,0.5 mg/L and 1.0 mg/L.The method is simple,convenient,sensitive,repeatable,stable and satisfactory.It 0is suitable for the accurate and rapid determination of abamectin in Chinese cabbage.HPLC with ultraviolet detector has a high popularity rate,the method has strong operability and basic promotion significance.