酱头抗胃溃疡的作用及机制研究

Protective effect of Fallopia denticuta against ethanol-induced gastric ulcer and its mechanism

目的:研究酱头对乙醇诱导的人胃上皮细胞(GES-1)的保护作用及其抗大鼠胃溃疡的作用和机制。方法:将细胞分为正常对照组、模型对照组、酱头95%醇提物组、酱头50%醇提物组、酱头水煎提取物组,采用1%乙醇建模,MTT法检测GES-1细胞增殖情况。60只SD大鼠随机分为正常对照组、模型对照组、雷尼替丁组,酱头95%醇提物小、中、大剂量组(150、300、600 mg/kg),连续灌胃14 do采用无水乙醇建模,比较各组大鼠胃溃疡面积及溃疡抑制率;扫描电镜观察胃黏膜损伤程度;酶联免疫吸附测定法检测血清中肿瘤坏死因子(TNF)-α、白介素(IL)-6,IL-1β水平,胃组织中丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、过氧化氢酶(CAT)活性。结果:三种酱头提取物中,酱头95%醇提物对于GES-1细胞的保护作用最强。动物实验中,与正常对照组比较,模型对照组胃黏膜上出现大量溃疡,而酱头95%醇提物各剂量组及雷尼替丁组溃疡面积较模型对照组明显减小(均P<0.05)。与模型对照组比较,酱头95%醇提物各剂量组及雷尼替丁组均可明显降低大鼠血清中TNF-α、IL-1β和IL-6水平及胃组织中MDA含量,提高胃组织中SOD、CAT、GSH活性(均P<0.05).结论:酱头95%醇提物可通过降低血清中TNF-α、IL-1β和IL-6水平及胃组织中MDA含量,提高胃组织中SOD、CAT、GSH活性,起到治疗乙醇诱导胃溃疡的作用。

Objective:To investigate the protective effect of Fallopia denticuta(FD)against ethanol-induced gastric ulcer and its mechanism.Methods:Human gastric epithelial GES-1 cells were divided into normal control group,model control group,FD 95%alcohol extract group,FD 50%alcohol extract group and FD decoction extract group.Gastric ulcer was induced by treatment with 1%ethanol in GES-1 cells.The cell proliferation was detected with MTT method in each group.Sixty SD rats were randomly divided into normal control group,model control group,ranitidine group and low-dose,medium-dose,high-dose FD 95%alcohol extract groups(150,300,600 mg/kg).The corresponding drugs were administrated by gavage for 14 d.The gastric ulcer model was induced by intragastric administration of anhydrous ethanol.The gastric ulcer area and ulcer inhibition rate of rats were measured in each group;the degree of gas trie mucosal damage was observed by scanning electron microscopy;the levels of tumor necrosis factor(TNF)-α,interleukin(IL)-6,IL-1β in serum and the content of malondialdehyde(MDA),superoxide dismutase(SOD),glutathione(GSH),catalase(CAT)in gastric tissues were detected by ELISA method.Results:95%alcohol extract of FD had the strongest protective effect on proliferation of GES-1 cells.In animal experiments,compared with the normal control group,a large area of ulcers appeared on the gastric mucosa in the model control group,while the ulcer areas of the FD groups and ranitidine group were significantly smaller than that of the model control group(all P<0.05).Compared with the model control group,FD groups and ranitidine group significantly reduced the levels of TNF-α,IL-1β,IL-6 in serum and the MDA content in the gastric tissues,and increased the activity o£SOD,CAT and GSH in gastric tissues(all P<0.05).Conclusion:The 95%alcohol extract of FD can reduce the levels of TNF-α,IL-1β and IL-6 in serum and the content of MDA in gastric tissues,and increase the activity of SOD,CAT and GSH in gastric tissues to achieve the protective effect against gastric ulcer.