miR-155在骨肉瘤发生发展及肺转移进程中的作用及机制

Role and Mechanism of miRNA-155 in Development,Progression and Lung Metastasis of Osteosarcoma

目的初步探究miR-155在骨肉瘤发生发展及其肺转移进程中的作用,并探讨其靶蛋白及相关机制。方法通过数据库分析,结合临床组织与细胞样本的相关检测,对骨肉瘤及其肺转移进程中miR-155差异性进行对比分析;应用iTRAQ定量蛋白质组学技术,筛选骨肉瘤及其肺转移进程中miR-155的靶蛋白;在临床组织与细胞样本中,对筛选的miR-155靶蛋白进行验证。结果miR-155在骨肉瘤组织和肺转移瘤组织中,较对照组均出现明显升高;与人正常成骨细胞相比,人骨肉瘤细胞系miR-155亦出现明显升高。利用iTRAQ实验得到3714个蛋白,筛选出差异表达蛋白253个,其中上调144个,下调109个。经预测分析和实验验证C/EBPβ是miR-155潜在的作用靶蛋白。结论miR-155在骨肉瘤及其肺转移进程中出现显著升高,C/EBPβ是其潜在的作用靶点。

Objective To investigate the role of miR-155 in the development and lung metastasis of osteosarcoma,and to explore its target proteins and related mechanisms.Methods We detected differential miR-155 expression in osteosarcoma and its lung metastasis process through relevant database analysis combined with qPCR detection of clinical tissue and cell samples.iTRAQ quantitative proteomics was used to screen the target protein of miR-155 in osteosarcoma and its lung metastasis.The selected miR-155 target protein was verified by Western blot in clinical tissue and cell samples.Results miR-155 expression was significantly higher in osteosarcoma tissue and lung metastatic tumor tissue than that in the control group.Compared with human normal osteoblasts,miR-155 expression in human osteosarcoma cell line was also significantly increased.A total of 3714 proteins were obtained by iTRAQ assay,and 253 differentiallyexpressed proteins were screened out,144 of which were up-regulated and 109 were down-regulated.The prediction analysis and experiment verified that C/EBPβwas a potential target protein of miR-155.Conclusion miR-155 expression is significantly increased in osteosarcoma and its lung metastasis process,and C/EBPβis a potential target of miR-155.