不同栽培密度与叶面肥喷施浓度对水稻叶片光系统Ⅱ荧光参数及SPAD值的影响

Effects of Different Planting Densities and Foliar Fertilizer Spraying Concentration on PhotosystemⅡFluorescence Parameters and SPAD Value of Rice Leaf

为探究不同栽培密度与叶面肥喷施浓度对水稻叶片光系统Ⅱ(Photosystem II,PSⅡ)荧光参数及SPAD值影响,并筛选出促进水稻光合作用的最佳肥密处理。本研究通过栽培密度及叶面肥喷施浓度裂区试验,利用植物多功能仪测定水稻分蘖期叶片水稻叶片PSⅡ的非光化学猝灭(Non-photochemical quenching,NPQt)、有效量子产量(Effective quantum yield,Phi2)、非调节性能量耗散的量子产量(The quantum yield of unregulated energy dissipation,PhiNO)、调节性能量耗散的量子产量(The quantum yield of regulatory energy dissipation,PhiNPQ)及SPAD值,对各指标数据进行多重比较。研究发现,主区因素栽培密度对NPQt造成显著性影响,副区因素叶面肥喷施浓度对NPQt造成显著性影响,对Phi2及PhiNPQ造成极显著性影响,栽培密度及叶面肥喷施浓度耦合作用对PhiNO造成显著性影响,对Phi2、PhiNPQ造成极显著性影响。随着栽培密度的增加,NPQt呈先下降后上升的趋势、Phi2呈先升高后降低的趋势、PhiNO现先持平后降低的趋势、PhiNPQ先降低后升高的趋势、SPAD呈先上升后降低的趋势。随着叶面肥喷施浓度的增加,NPQt与PhiNPQ呈先增加后减少的趋势、Phi2与SPAD呈递增趋势、PhiNO呈先减少后增加的趋势。在栽培密度及叶面肥喷施浓度交互作用影响下,D4L2处理对水稻叶片的NPQt提升效果最佳,D3L3处理对水稻叶片的Phi2与PhiNO影响效果最佳,D4L3处理对水稻叶片的PhiNPQ提升效果最佳,D1L1处理的水稻叶片的SPAD值最高。综合比较筛选出D4L2处理为最优的栽培措施,可有效增强水稻的光合作用能力。研究表明不同的栽培密度与叶面肥喷施浓度对水稻叶片PSⅡ荧光参数造成的影响不同,利用不同处理下的水稻叶片的光合特性,可以为筛选合理的水稻栽培措施提供依据。

The aim of this study was to explore the effects of different planting densities and foliar fertilizer spraying concentration on the PSⅡfluorescence parameters and SPAD values of rice leaves to screen out the best planting density and foliar fertilizer concentration to promote rice photosynthesis.Through split plot experiment on cultivation density and foliar fertilizer spraying concentration,Non-photochemical quenching(NPQT),effective quantum yield(Phi2),quantum yield of non-regulatory energy dissipation(PhiNO),quantum yield of regulatory energy dissipation(PhiNPQ)and SPAD values of PSⅡin rice leaves at tillering stage were measured by plant multifunctional apparatus,and multiple comparison was made for the data of each index.The results showed that the planting density of the main factor had a significant impact on NPQt;the foliar fertilizer spraying concentration of the secondary factor had a significant impact on NPQt,and had highly significant effects on Phi2 and PhiNPQ;the coupling action of planting density and foliar fertilizer spraying concentration had a significant impact on PhiNO,and extremely significant impact on Phi2 and PhiNPQ.With the increase of planting density,NPQt first decreased and then increased,Phi2 first increased and then decreased,PhiNO first kept invariant and then decreased,PhiNPQ first decreased and then increased,and SPAD first increased and then decreased.With the increase of foliar fertilizer spraying concentration,NPQt and PhiNPQ first increased and then decreased,Phi2 and SPAD increased gradually,and PhiNO first decreased and then increased.Under the interaction effects of planting density and foliar fertilizer spraying concentration,D4L2 treatment had the best effect on NPQt,D3L3 treatment had the best effect on Phi2 and PhiNO,D4L3 treatment had the best effect on PhiNPQ,and D1L1 treatment had the highest SPAD value.Through comprehensive comparison,D4L2 treatment was selected as the best cultivation measure,which could effectively enhance the photosynthetic capacity in rice leaves.The results showed that different planting density and foliar fertilizer spraying concentration had different effects on the fluorescence parameters of PSⅡin rice leaves.Analysis of the photosynthetic characteristics in rice leaves under different treatments will provide a basis for screening reasonable cultivation measures of rice.