基于GC-MS分析不同品种小米代谢产物及代谢途径

Analysis of Metabolites and Metabolic Pathways in Different Varieties of Foxtail Millets(Setaria italica L.)Based on Gas Chromatography-Mass Spectrometry

基于气相色谱-质谱(gas chromatography-mass spectrometry,GC-MS)代谢组学方法研究同一产地不同品种小米的代谢产物。用甲醇溶液提取极性代谢组分,N,O-双(三甲基硅基)三氟乙酰胺衍生化处理,通过GC-MS分离鉴定代谢产物。结果表明,同一产地2个不同品种小米共发现了53种代谢产物。古龙小米有48种代谢产物,其中优势代谢产物为亚油酸;禾绅小米代谢产物有32种,优势代谢产物为亚油酸。对比发现,古龙小米有21种差异代谢产物;禾绅小米有5种差异代谢产物。对2个品种小米差异代谢产物的代谢途径分析,发现古龙小米的葡萄糖代谢途径、脂肪酸代谢途径、氨基酸代谢途径和三羧酸循环都比禾绅小米对应的代谢途径更活跃,推测古龙小米的代谢产物多是因为其代谢速率快于禾绅小米的代谢速率。

The metabolites in two different varieties of foxtail millets from the same geographical origin were studied by metabonomics based on gas chromatography-mass spectrometry(GC-MS).The polar metabolites were extracted using methanol solution and then derivatized with N,O-bis(trimethylsilyl)trifluoroacetamide prior to analysis by GC-MS.The results showed that a total of 53 metabolites were identified,48 of which were found in‘Gulong’millet,the dominant ones being linoleic acid and 2-linolacyl-rac-glycerol;and 32 in‘Heshen’millet,the dominant ones being linoleic acid and glycerol.There were 21 differential metabolites identified in‘Gulong’millet and five differential metabolites in‘Heshen’millet.The metabolic pathways of the differential metabolites were also studied.It was found that the glucose,fatty acid,amino acid metabolism pathways and the tricarboxylic acid cycle in‘Gulong’millet were more active than those in Hesen millet.It was speculated that the increase in the number of metabolites in‘Gulong’millet may be because its metabolic rate was faster than that of‘Heshen’millet.