西瓜籽油脂肪酸成分分析及抗氧化活性研究

Fatty acid composition analysis and antioxidantactivity of watermelon seed oil

目的:研究西瓜籽油脂肪酸成分及抗氧化活性。方法:采用索氏回流法对西瓜籽进行提油,对西瓜籽油进行酯化处理,用气相色谱—质谱联机(GC-MS)对其脂肪酸的成分、含量进行分析,通过其对铁离子的还原能力和对DPPH自由基、羟基自由基及超氧阴离子自由基清除能力的测定。结果:西瓜籽油中含有6种脂肪酸,其中不饱和脂肪酸相对含量达到50%以上;西瓜籽油对铁离子具有还原能力,对DPPH自由基的IC_(50)值为3.7 mg/mL,对超氧阴离子自由基的IC_(50)值为0.031 mg/mL,对羟基自由基的IC_(50)值为12.04 mg/mL。结论:西瓜籽油富含不饱和脂肪酸,有较好的抗氧化活性。

Objective:This study focused on the fatty acid composition and antioxidant activity of watermelon seed oil.Methods:The watermelon seed oil was extracted by Soxhlet extraction from the watermelon seed,then the watermelon seed oil was esterified.Thereafter,the components and the contents of watermelon seed oil were analyzed and detected used the method of gas chromatography-mass spectrometry(GC-MS),and the antioxidant activity of watermelon seed oil was studied by measuring its ability to reduce iron ions and scavenge DPPH radical,hydroxyl radical and superoxide anion.Results:The results of GC-MS showed that there were 6 fatty acids in watermelon seed oil,the relative content of unsaturated fatty acidswas more than 50%,the study of antioxidant activity showed that watermelon seed oil had the ability to reduce iron ions,and the IC_(50) value of watermelon seed oil for eliminating DPPH free radical,superoxide anion and hydroxyl free radicals was 3.7 mg/mL,0.031 mg/mL and 12.04 mg/mL,respectively.Conclusion:Watermelon seed oil is rich in unsaturated fatty acids and has good antioxidant activity.