间氨基苯甲酸乙酯甲磺酸盐在大菱鲆模拟保活运输中的作用效果

Effect of 3-Aminobenzoic Acid Ethyl Ester Methanesulfonate(MS-222)on Improving the Survival of Turbot(Scophthalmus maximus)during Simulated Live Transportation

研究间氨基苯甲酸乙酯甲磺酸盐(3-aminobenzoic acid ethyl ester methanesulfonate,MS-222)对大菱鲆的麻醉效果,并进行模拟运输实验,研究大菱鲆在不同质量浓度的MS-222麻醉液、运输水温及鱼水质量比(简称鱼水比)中的存活率,筛选出大菱鲆麻醉保活运输的最优条件,为大菱鲆的保活运输提供参考。在静水条件下,研究MS-222质量浓度(20、40、60、80、100 mg/L和120 mg/L)对大菱鲆行为的影响,测定其麻醉效果、复苏时间及复苏存活率,确定MS-222对大菱鲆的有效麻醉质量浓度。在此条件下,分别设定不同质量浓度的MS-222(20、40、60、80 mg/L)麻醉液、不同的水温(2、8、13、20℃)以及不同鱼水比(2∶1、1∶1、1∶3、1∶5)进行模拟运输,记录大菱鲆的存活率,在运输不同时间段,对运输水体及大菱鲆进行取样,检测运输过程中水体氨氮和溶解氧水平的变化、大菱鲆生理生化指标。结果表明:水温为8℃、MS-222质量浓度为40 mg/L、鱼水比为1∶3时,大菱鲆保活运输时间长,适合大菱鲆长时间(24 h)运输;随运输时间延长,水中氨氮质量浓度升高,溶解氧水平降低;在鱼体肌肉指标中,随运输时间的延长,乳酸含量上升,糖原含量、pH值呈下降趋势;在大菱鲆血清生化指标中,乳酸脱氢酶、谷草转氨酶、血糖、尿素、肌酐水平在运输24 h过程中均变化显著(P<0.05),这表明大菱鲆肝脏、肾功能在运输过程中受到一定损伤,麻醉组鱼体生化指标的变化均小于对照组。综上,适当使用MS-222可以提高大菱鲆保活运输的存活率,延长保活运输时间。

In order to study the anesthetic effect of 3-aminobenzoic acid ethyl ester methanesulfonate(MS-222)in turbot,simulated transportation experiments were conducted to evaluate the survival rate of turbot under varying conditions of MS-222 concentration(20,40,60 and 80 mg/L),water temperature(2,8,13 and 20℃)and fish to water ratio(2:1,1:1,1:3 and 1:5;m/m),and the optimal live transportation conditions were determined.Before this,under still water conditions,the influence of different MS-222 concentrations(20,40,60,80,100 and 120 mg/L)on the behavior of turbot was explored by determining the anesthesia time,recovery time and survival rate after recovery to determine the effective anesthetic concentration of MS-222 for turbot.Meanwhile,the changes in the ammonia nitrogen concentration in the water and the changes in physiological and biochemical indicators in the serum and muscle of turbot were measured during simulated live transportation.Results showed that a water temperature of 8℃,an MS-222 concentration of 40 mg/L and a fish to water ratio of 1:3 were suitable for long-time live transportation(24 h)of turbot.With increasing transportation time,the ammonia nitrogen concentration in the water increased and the dissolved oxygen level decreased.The lactic acid content in the muscle increased,and the glycogen content and pH decreased.The activities of lactate dehydrogenase and aspartate aminotransferase and the levels of glucose,urea and creatinine in the serum changed significantly with transportation time up to 24 hours(P<0.05),indicating that the liver and kidney functions of turbot were damaged during transportation,and the changes in all biochemical indexes were smaller in the anesthesia group than in the control group.Taken together,the proper application of MS-222 can increase the survival rate of turbot and prolong the live transportation time.