蒽醌暴露对秀丽隐杆线虫的毒性作用

Toxic effects of exposure to anthraquinone on the C.elegans

目的研究蒽醌暴露对模式生物秀丽隐杆线虫的毒性作用,观察其对秀丽隐杆线虫急性致死效应、寿命、生殖能力及抗氧化酶的影响。方法根据蒽醌的溶解度及急性毒性试验结果确定本研究剂量为0、8、20、50、125μmol/L,另设1%二甲基甲酰胺(DMF)溶剂对照组,对同步化至L4期的秀丽隐杆线虫分别进行处理,测定其致死率、寿命、子代数目及抗氧化酶的变化。结果蒽醌24 h急性暴露后,随剂量的增加,秀丽隐杆线虫致死率增加,20、50、125μmol/L剂量组与1%DMF溶剂对照组相比差异均有统计学意义(P<0.05或P<0.01);8、20、50、125μmol/L剂量组与1%DMF溶剂对照组相比,超氧化物歧化酶(SOD)活力增加(均P<0.01)。蒽醌长期暴露后,125μmol/L剂量组与1%DMF溶剂对照组相比子代数目减少(P<0.05);20、50、125μmol/L剂量组与1%DMF溶剂对照组相比平均寿命明显减少(P<0.05或P<0.01)。结论秀丽隐杆线虫暴露于蒽醌可对其致死率、寿命、生殖能力产生影响,并可激活其体内抗氧化酶,启动氧化防御系统。

Objective To study the toxic effects of exposure to anthraquinone on acute lethal effect,life span,fecundity and activity of antioxidant enzymes in Caenorhabditis elegans(C.elegans).Methods Nematodes at L4 stage were exposed to anthraquinone(with the concentration of 0,8,20,50,125μmol/L respectively based on the solubility of anthraquinone and acute toxicity test)and 1%dimethylformamide(DMF),the lethality,life span,brood size and activity of enzymes were measured.Results After 24 hours of acute exposure to anthraquinone,the nematode lethality of C.elegans increased with the increase of the dose,with the concentration of 20、50、125μmol/L,the lethality rates were statistically different compared with the 1%DMF group(P<0.05 or P<0.01).Meanwhile,the exposure of 8,20,50,125μmol/L,the increased activities of superoxide(SOD)were detected(P<0.01).After long-term exposure to anthraquinone,compared with the 1%DMF group,exposure with the concentration of 125μmol/L induced the reduction of brood size(P<0.05).The average lifespan was decreased at the concentrations of 20,50,and 125μmol/L(P<0.05 or P<0.01).Conclusion Exposure to anthraquinone can affect the lethality rate,lifespan and fecundity of C.elegans.In addition,anthraquinone can activate antioxidant enzymes and the oxidative defense system.